This Grin2bflox CRISPR/Cas9 generated mutant of the Grin2b gene possesses loxP sites flanking 599 nucleotides (exon 4 of Ensembl transcript 201 and exon 5 of transcript 202). This strain may be useful for generating conditional mutations in applications related to neurodevelopment.
Cathleen Lutz, The Jackson Laboratory
CRISPR/cas9 endonuclease mediated genome editing of the Grin2b, glutamate receptor, ionotropic, NMDA2B (epsilon 2), gene was used to introduce loxP sites flanking 599 nucleotides (exon 4 of Ensembl transcript 201 and exon 5 of transcript 202). The targeted Grin2b gene encodes part of the excitatory neurotransmitter N-methyl-D-aspartate (NMDA) receptor. Mutations in this gene have been associated with Mental Retardation, Autosomal Dominant 6, with or without Seizures and Early Infantile Epileptic Encephalopathy 27. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have loxP site flanked region deleted in the cre-expressing tissues. As the mice are characterized, we will modify the strain description and add phenotype data.
This model was generated in collaboration with the Simons Foundation Autism Research Initiative (SFARI).
Two guide RNAs were synthetized, a double stranded DNA donor plasmid containing loxP sequences flanking the second coding exon of Grin2b (exon 4 of Ensembl Grin2b transcript 201/ exon 5 of Ensembl transcript 202), and cas9 nuclease were co-injected into the cytoplasm of single cell C57BL/6J-derived zygotes containing well recognized pronuclei. Correctly targeted embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by DNA sequencing of the targeted exon and flanking introns and further bred to C57BL/6J (Stock# 000664) to develop the colony. Mice derived from founder 1087 (GET2481) were further bred to C57BL/6J inbred mice for 2 generations to develop the colony.
|Allele Name||endonuclease-mediated mutation 5, Cathy Lutz|
|Allele Type||Endonuclease-mediated (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||endonuclease-mediated mutation 5, Cathy Lutz; Grin2bem5Lutzy|
|Gene Symbol and Name||Grin2b, glutamate receptor, ionotropic, NMDA2B (epsilon 2)|
|Gene Synonym(s)||GluRepsilon2; Nmdar2b; NMDAR2B; NR2B; MRD6; GluN2B; AW490526; expressed sequence AW490526; EIEE27; Nmdar2b; NR3; hNR3|
|Strain of Origin||C57BL/6J|
|Molecular Note||CRISPR/Cas9 genome editing is used to insert loxP sequences flanking the second coding exon of Grin2b (exon 4 of Ensembl Grin2b transcript 201/ exon 5 of Ensembl transcript 202).|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Grin2b flox
mouse strain in a publication, please cite the originating article(s) and include JAX stock #032664 in your Materials and Methods section.
|Heterozygous for Grin2b<em5Lutzy>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.