These Irf4 knock-out mice exhibit reduced serum immunoglobulin levels and increased susceptibility to viral and parasitic infection. They are suitable for use in applications related to the study of B cell function and T helper (Th) cell differentiation.
Dr. Tak Mak, University Health Network/Un of Toronto
The targeted Irf4 gene encodes the lymphocyte-specific interferon regulatory
factor. Mutations in this gene have been associated with chronic lymphocytic leukemia. These mice carry a knockout allele of Irf4, in which exons 2 and 3 have been replaced by a NEO cassette. No gene product (protein) is detected by western blot analysis of concanavalin A activated lymph nodes from homozygous animals. Mice that are homozygous for this allele exhibit enlarged spleens and lymph nodes due to increased number of T and B lymphocytes, decreased serum immunoglobulins levels, and reduced T and B cell proliferation.
Germinal centers in spleens and lymph nodes are absent. Lymphocytic
choriomeningitis virus (LCMV) challenge does not result in expected immunological response (cytotoxic T
Homozygotes are more susceptibility to Leishmania major infection.
Homozygotes are viable and fertile.
A targeting vector containing a PGK-NEO (pKJ1) cassette was used to disrupt exons 2 and 3.
The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into CD1 blastocysts.
The resulting chimeric male animals were crossed to C57BL/6 female mice. The mice were then backcrossed to C57BL/6 for 10 generations.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Allele Name||targeted mutation 1, Tak Mak|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, Tak Mak; Irf4tm1Mak|
|Gene Symbol and Name||Irf4, interferon regulatory factor 4|
|Gene Synonym(s)||NF-EM5; Sfpi1/PU.1 interaction partner; MUM1; AI385587; IRF-4; Spip; Spip; expressed sequence AI385587; LSIRF; SHEP8|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exons 2 and 3 were replaced by a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of concanavalin A activated lymph nodes obtained from homozygous mutant mice.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Irf4 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #031834 in your Materials and Methods section.
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