Expression of the human MAPT, hTau-WT (wildtype) variant, is regulated by the tetracycline-responsive regulatory element (tetO) in these mice. When mated with a strain expressing tetracycline-controlled transactivator protein (tTA), expression of the hTau protein may be regulated with the tetracycline analog doxycycline (dox) in the double-allelic offspring. This strain may be useful as a control strain for (Stock No. 028979) and in studies related to frontotemporal dementia, Alzheimer’s disease, and other neurodegenerative diseases.
Lennart Mucke, Gladstone Inst of Neurological Disease
Mice hemizygous for the hTau-WT transgene are viable and fertile. Expression of the 1N4R isoform of human MAPT (microtubule associated protein tau) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator). When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of 1N4R isoform of human MAPT protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. The Donating Investigator reports no human MAPT protein is detected in brain sections from singly transgenic mice by western blotting and immunohistochemical staining. Hemizygotes are viable and fertile. Homozygous viability/fertility has not been tested (June, 2016).
This strain may serve as a control strain for (Stock No. 028979).
When these line 32 hTau-WT transgenic mice are crossed to CaMKII-tTA mice (see, for example, Stock No. 003010), the double mutant mice express a similar level of the human MAPT protein when compared to C11-1 line hTau-A152T transgenic mice (Stock No. 028979).
A transgenic construct containing cDNA of the human MAPT gene encoding the 1N4R isoform, with upstream intronic sequence (from pCI-neo vector) and a Kozak sequence, and downstream bovine growth hormone polyadenylation sequence, all under the control of the tetO, tetracycline-responsive regulatory element (TRE-tight), was injected into fertilized C57BL/6 mouse eggs. Founder line 32, was subsequently established. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
|Expressed Gene||MAPT, microtubule associated protein tau, human|
|Site of Expression|
|Allele Name||transgene insertion 32, Lennart Mucke|
|Allele Type||Transgenic (Inducible, Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||hTau-WT; TRE-hTau-WT|
|Gene Symbol and Name||Tg(tetO-MAPT)32Lms, transgene insertion 32, Lennart Mucke|
|Promoter||tetO, tet operator,|
|Expressed Gene||MAPT, microtubule associated protein tau, human|
|Strain of Origin||C57BL/6|
|Molecular Note||A transgenic construct containing cDNA of the human MAPT gene encoding the 1N4R isoform, with upstream intronic sequence (from pCI-neo vector) and a Kozak sequence, and downstream bovine growth hormone polyadenylation sequence, all under the control of the tetO, tetracycline-responsive regulatory element (TRE-tight).|
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.
When using the TRE-hTau-WT line 32 mouse strain in a publication, please cite the originating article(s) and include JAX stock #029269 in your Materials and Methods section.
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