C57BL/6J-Card14^em5Lutzy/J

Stock number: 028882
Product name: Card14ΔE138 secondary line
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

This CRISPR/Cas9 generated mutant of the Card14 gene carries a deletion of the glutamic acid amino acid (E) at position 138. These mice may be suitable for use in studies related to the IL-23/IL-17 axis in inflammation.

The Jackson Laboratory Repository distributes another (primary) strain carrying the same Card14ΔE138 mutation: (Stock No. 028900).

Detailed description

This CRISPR/Cas9 generated point mutant of the Card14 (caspase recruitment domain family, member 14 ), carries deletion of a specific GAG codon from exon 6 that results in the deletion of the glutamic acid (E) at position 138. The Card14 gene encodes a scaffold protein that is a regulator of apoptosis and may have a role in innate immunity. Mutations in this gene, specifically of the single glutamic acid (E138) in the coiled-coil domain, have been associated with psoriasis and pityriasis rubra pilaris. As the mice are characterized, we will modify the strain description and add phenotype data. Homozygous viability/fertility has not been tested (June 2018).

Creation and development of this strain was supported by the Rare Genomics Institute's BeHEARD(Helping Empower and Accelerate Research Discoveries) Initiative. The Jackson Laboratory was a 2016/2017 BeHEARD Technology Sponsor.

Development

Plasmids encoding a single guide RNA designed to delete a specific GAG codon from exon 6 in the Card14 gene and the cas9 nuclease were introduced into the cytoplasm C57BL/6J-derived fertilized eggs with well recognized pronuclei. Correctly targeted embryos were transferred to pseudopregnant females. Correctly targeted pups were identified by sequencing and PCR and further bred to C57BL/6J (Stock No. 000664) to develop the colony.

Allele

Symbol: Card14^em5Lutzy
Name: endonuclease-mediated mutation 5, Cathy Lutz
MGI accession ID: MGI:6164594
Generation method: Endonuclease-mediated
Attributes: Humanized sequence
Marker symbol: Card14
Marker name: caspase recruitment domain family, member 14
Chromosome: 11
Strain of origin: C57BL/6J
Molecular note: CRISPR/cas9 endonuclease mediated genome editing was used to introduce a deletion of a GAG codon from exon 6 that results in the deletion of the glutamic acid (E) at position 138. Card14^em5Lutzy and Card14^em9Lutzy were generated from two different founders, both carry the same mutation.