MybF/F floxed mutant mice possess loxP sites flanking exon 6 of the myeloblastosis oncogene gene. This strain may be useful for studying the proliferation and differentiation of hematopoietic progenitor cells.
E. P. Reddy, Mount Sinai School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Myb | myeloblastosis oncogene |
MybF/F floxed mutant mice possess loxP sites flanking exon 6 of the myeloblastosis oncogene (Myb). MYB is a transcription factor involved in the proliferation and differentiation of hematopoietic progenitor cells. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 6 deleted in cre-expressing tissues.
When crossed to B6.Cg-Tg(Lck-cre)548Jxm/J mice (Stock No. 003802) expressing Cre recombinase T lymphocytes, T cells in resulting mybF/F/LckCre mice did not progress from the DN3 stage to the DN4 stage of thymocyte development. Thymic cellularity is decreased to 11% of that seen in controls. Double positive (DP) thymocytes are reduced by 62% compared to littermate controls.
When crossed to B6.Cg-Tg(Mx1-cre)1Cgn/J mice (Stock No. 003556) expressing Cre recombinase in adult bone marrow, resulting mybF/F/MxCre mice have reductions of hematopoietic lineages including neutrophilic, monocytic, B lymphoid, erythroid, and, megakaryocytic cells after polyinosinic-polycytidylic acid (pIpC) administration.
Stock No. 035170, a similar strain with loxP sites flanking exon 2, takes into account that splicing of nascent Myb mRNA between exon 1 and 2 occurs in the first reading frame, whereas all other known splices between coding exons are in the second and third reading frames. Thus, there
are no known splicing events that could occur in frame down stream of exon 1 after Cre mediated deletion of exon 2. c-Myb proteins are not detected using antibodies that
recognize epitopes in the N- or C-terminal ends after cre recombination in this line.
This Stock No. 028881 with exon 6 floxed, targets the c-Myb DNA binding domain, rather than exon 2. Due to a variety of c-My splice variants expressed from different promoters, deletion of exon 6 may still produce a truncated protein driven by a promoter that is still present upstream of exon 2. This includes 2 major variants that involve exons 9 and 9A.
A targeting vector was designed to insert a loxP-flanked neomycin resistance (neo) cassette upstream of exon 6, and a single loxP site downstream of exon 6 of the myeloblastosis oncogene (Myb) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pPGKcre-bpA expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exon 6, intact floxed-neo cassette, or excision of both exon 6 and the neo cassette. Correctly targeted ES cells, containing only the floxed-exon 6, were injected into C57BL/6 x DBA2 blastocysts and resulting chimeric males were bred with C57BL/6J females. Resulting MybF/F offspring were bred to C57BL/6J mice for at least 10 generations. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1, E Premkumar Reddy |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | mybF |
Gene Symbol and Name | Myb, myeloblastosis oncogene |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 10 |
Molecular Note | Exon 6 encoding the high-affinity DNA-binding domain was flanked with loxP sites by homologous recombination of a construct that also contained a PGK-neo and thymidine kinase selection markers. The PGK-neo-thymidine kinase marker was excised by transient transfection of properly targeted ES cells with a cre-expressing plasmid and left exon 6 flanked by loxP sites. |
When maintaining a live colony mice homozygous for the floxed allele may be bred together.
When using the MybF mouse strain in a publication, please cite the originating article(s) and include JAX stock #028881 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Myb<tm1Epr> |
Frozen Mouse Embryo | B6.129-Myb<tm1Epr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Myb<tm1Epr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Myb<tm1Epr>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Myb<tm1Epr>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.