Exons 2-4 of the mouse Wrb gene are flanked by loxP sites in this targeted mutant strain. Cre-mediated excision of the floxed region is believed to result in a knockout allele. The human homolog maps to Chromosome 21q22.3 in a region associated with Down Syndrome. Mutations in the zebrafish homolog have been shown to result in blindness and deafness.
David P. Corey, Harvard Medical School/HHMI
Wrb (tryptophan rich basic protein) is broadly expressed in both adult and embryonic tissues, but its function remains largely uncharacterized. The human homolog maps to Chromosome 21q22.3 in a region associated with Down Syndrome. Mutations in the zebrafish homolog have been shown to result in blindness and deafness.
Exons 2-4 of the mouse Wrb gene are flanked by loxP sites in this targeted mutant strain. Cre-mediated excision of the floxed region is believed to result in a knockout allele.
A loxP site was inserted 5' to exon 2, and a loxP- and FRT-flanked neomycin cassette was placed 3' to exon 4. The mutation was created through homologous recombination in (C57BL/6NTac x 129S6/SvEvTac)F1-derived BA1 embryonic stem (ES) cells. Resultant mice were crossed with Tg(ACTFLPe)9205Dym mice to excise the FRT-flanked neomycin resistance cassette, leaving exons 2-4 flanked by loxP sites. Animals were crossed with a Slc17a8 (Vglut3)-cre recombinase-expressing strain on a mixed 129-C57BL/6-FVB background by the donating laboratory, but the cre allele was bred out prior to arrival at The Jackson Laboratory. Exons 2-4 remain flanked by loxP sites.
|Allele Name||targeted mutation 1.1, David P Corey|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1.1, David P Corey; Wrbtm1.1Dpc|
|Gene Symbol and Name||Wrb, tryptophan rich basic protein|
|Gene Synonym(s)||C030018G21Rik; 5530402J05Rik; 5530402J05Rik; CHD5; C030018G21Rik; RIKEN cDNA C030018G21 gene; RIKEN cDNA 5530402J05 gene; Chd5; GET1|
|Strain of Origin||(C57BL/6NTac x 129S6/SvEvTac)F1|
|General Note||ES cell line BA1|
|Molecular Note||A loxP site was inserted 5' to exon 2, and a loxP- and FRT-flanked neomycin cassette was placed 3' to exon 4. The FRT-flanked neomcycin cassette was deleted by crossing the mice with a widely-expressed Flp strain. To create this allele (delta P), the floxed exons were also excised by crossing to a widely expressing Cre strain, leaving a single loxP site.|
Homozygous and heterozygous floxed mice are viable and fertile.
When using the Wrb conditional knock-out mouse strain in a publication, please cite the originating article(s) and include JAX stock #028762 in your Materials and Methods section.
|Heterozygous for Wrb<tm1.1Dpc>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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