NOD.Cg-Prkdc^scid Il2rg^tm1Wjl Tg(CSF1)3Sz/SzJ

Stock number: 028654
Product name: NSG CSF1
Strain synonyms: NSG-CSF1
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

NSG CSF1 mice express human CSF1 [colony stimulating factor 1 (macrophage)] on the immunodeficient NSG background. In humanized NSG CSF1 mice, the human CSF1 protein improves the function of human macrophages and monocytes.

Detailed description

NSG CSF1 mice express human CSF1 [colony stimulating factor 1 (macrophage)] on the immunodeficient NSG (Stock No. 005557) background. NSG mice carry the Prkdc^scid and Il2rg^tm1Wjl alleles on the NOD/LtShiJ background; they should have no mature T cells or B cells, lack functional natural killer (NK) cells, have reduced numbers of lymphocytes and myeloid dendritic cells, and are deficient in cytokine signaling. CSF1 encodes a cytokine involved in the regulation of macrophages. The NSG CSF1 strain is used to make "humanized mice", which are created by injecting human immune cells. The CSF1 protein improves the function of human macrophages and monocytes that develop in the humanized mouse. In turn, the macrophages and monocytes assist other immune cells such as Natural Killer cells.

Development

A transgenic construct containing the human colony stimulating factor 1 (macrophage), CSF1, gene from the 157 Kb BAC clone RP11-19F3 was microinjected into the pronuclei of fertilized NOD.CB17-Prkdc^scid/J eggs. Founder line 3 was established and mated to NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/J (Stock No. 005557) mice. Progeny were interbred until all offspring were homozygous for scid, hemizygous/homozygous for the Il2rg targeted mutation and hemizygous for the transgene.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Allele

Symbol: Il2rg^tm1Wjl
Name: targeted mutation 1, Warren J Leonard
MGI accession ID: MGI:1857455
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il2rg
Marker name: interleukin 2 receptor, gamma chain
Chromosome: X
Strain of origin: 129S4/SvJae
Site of expression: Primarily lymphoid cells.
Molecular note: A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.

Allele

Symbol: Tg(CSF1)3Sz
Name: transgene insertion 3, Leonard Shultz
MGI accession ID: MGI:5699722
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(CSF1)3Sz
Marker name: transgene insertion 3, Leonard Shultz
Chromosome: UN
Strain of origin: NOD.Cg-Prkdc^scid
Expressed genes: CSF1,colony stimulating factor 1,human
Site of expression: CSF1 encodes a cytokine involved in the regulation of macrophages.
Molecular note: This transgene consists of a 157 kb human BAC clone, RP11-19F3, containing the entire genomic sequence of the human CSF1 gene including the endogenous promoter. CSF1 is the only gene in the plasmid.