Overview

Also Known As:NSG CSF1, NSG-CSF1

NSG CSF1 mice express human CSF1 [colony stimulating factor 1 (macrophage)] on the immunodeficient NSG background. In humanized NSG CSF1 mice, the human CSF1 protein improves the function of human macrophages and monocytes.

Donating Investigator

Dr. Leonard D. Shultz, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
	?+pN1F6 (2020-06-25 00:00:00)

Prkdc^scid

Allele Type	Gene Symbol	Gene Name
Spontaneous	Prkdc	protein kinase, DNA activated, catalytic polypeptide

Il2rg^tm1Wjl

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Il2rg	interleukin 2 receptor, gamma chain

Tg(CSF1)3Sz

Allele Type
Transgenic (Inserted expressed sequence, Humanized sequence)

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Research Tools
Internal/Organ Research
Virology Research
Cancer Research

View All Research Applications

Base Price

Starting at:

$275.89 Domestic price for male 4-week

287.24 Domestic price for breeder pair

View Price List

Details

Detailed Description

NSG CSF1 mice express human CSF1 [colony stimulating factor 1 (macrophage)] on the immunodeficient NSG (Stock No. 05557) background. NSG mice carry the Prkdc^scid and Il2rg^tm1Wjl alleles on the NOD/LtShiJ background; they should have no mature T cells or B cells, lack functional natural killer (NK) cells, have reduced numbers of lymphocytes and myeloid dendritic cells, and be deficient in cytokine signaling. CSF1 encodes a cytokine involved in the regulation of macrophages. The NSG CSF1 strain is used to make "humanized mice", which are created by injecting human immune cells. The CSF1 protein improves the function of human macrophages and monocytes that develop in the humanized mouse. In turn, the macrophages and monocytes assist other immune cells such as Natural Killer cells.

View our Resources for the NSG mouse model, including discussion forum, immunodeficient model comparison, and categorized, up-to-date references.

Development

A transgenic construct containing the human colony stimulating factor 1 (macrophage), CSF1, gene from the 157 Kb BAC clone RP11-19F3 was microinjected into the pronuclei of fertilized NOD.CB17-Prkdc^scid/J eggs. Founder line 3 was established and mated to NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/J (Stock No. 005557) mice. Progeny were interbred until all offspring were homozygous for scid, hemizygous/homozygous for the Il2rg targeted mutation and hemizygous for the transgene.

Expression Data

Expressed Gene	CSF1, colony stimulating factor 1, human
Site of Expression	CSF1 encodes a cytokine involved in the regulation of macrophages.

Control Suggestions

Additional Information

Considerations for Choosing Controls

Selected References

Lee J; Brehm MA; Greiner D; Shultz LD; Kornfeld H. 2013. Engrafted human cells generate adaptive immune responses to Mycobacterium bovis BCG infection in humanized mice. BMC Immunol 14:53PubMed: 24313934 MGI: J:284897

View All References

Genetics

Prkdc^scid

Allele Symbol: Prkdc^scid

Allele Name	severe combined immunodeficiency
Allele Type	Spontaneous
Allele Synonym(s)	SCID
Gene Symbol and Name	Prkdc, protein kinase, DNA activated, catalytic polypeptide
Gene Synonym(s)
Site of Expression	T and B lymphocytes.
Strain of Origin	C.BKa-Igh^b/Icr
Chromosome	16
Molecular Note	A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Allele Name	targeted mutation 1, Warren J Leonard
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	[KO]gammac; CD132^-; gammac^-; gc^-; Il2rg^tm1Wjll; IL2Rgamma^null
Gene Symbol and Name	Il2rg, interleukin 2 receptor, gamma chain
Gene Synonym(s)
Site of Expression	Primarily lymphoid cells.
Strain of Origin	129S4/SvJae
Chromosome	X
Molecular Note	A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.
Mutations Made By	Dr. Warren Leonard, NHLBI, NIH

Tg(CSF1)3Sz

Allele Symbol: Tg(CSF1)3Sz

Allele Name	transgene insertion 3, Leonard Shultz
Allele Type	Transgenic (Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)
Gene Symbol and Name	Tg(CSF1)3Sz, transgene insertion 3, Leonard Shultz
Gene Synonym(s)
Promoter	CSF1, colony stimulating factor 1, human
Expressed Gene	CSF1, colony stimulating factor 1, human
Site of Expression	CSF1 encodes a cytokine involved in the regulation of macrophages.
Strain of Origin	NOD.Cg-Prkdc^scid
Chromosome	UN
Molecular Note	This transgene consists of a 157 kb human BAC clone, RP11-19F3, containing the entire genomic sequence of the human CSF1 gene including the endogenous promoter. CSF1 is the only gene in the plasmid.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Il2rg^tm1Wjl related

Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines
Cancer Research
- Growth Factors/Receptors/Cytokines

Genotype: Prkdc^scid related

Research Tools
- Toxicology Research
  - xenograft/transplant host
- Cancer Research
  - B and T cell deficiency, xenograft/transplant host
Internal/Organ Research
- Lymphoid Tissue Defects
  - B and T cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B and T cell deficiency
Virology Research
- B and T Cell Deficiency
  - AIDS research tool

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - B, T, and NK cell deficiency
Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - B, T, and NK cell deficiency

Mammalian Phenotype Terms by Genotype

References

Lee J; Brehm MA; Greiner D; Shultz LD; Kornfeld H. 2013. Engrafted human cells generate adaptive immune responses to Mycobacterium bovis BCG infection in humanized mice. BMC Immunol 14:53PubMed: 24313934 MGI: J:284897

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related

Additional - Tg(CSF1)3Sz related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(CSF1)3Sz
- QPCR:Tg(CSF1)3Sz-qPCR
- Probe:Il2rg-PROBE
- End Point Analysis:Prkdc End Point
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice are bred as Hom (scid) Hom (Il2rg) Noncarrier (no CSF1 transgene) x Hom (scid) Hemi (Il2rg) Hemi (CSF1 transgene) or reciprocal. Il2rg is located on the X chromosome. Males homozygous for the transgene do not produce viable sperm.

This strain should be housed under pathogen free conditions similar to the Prkdc^scid/Prkdc^scid mouse or any other immunodeficient strain.
- Additional Breeding and Husbandry Support
Mating System
- See "Breeding Considerations"
Citation
When using the NSG CSF1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #028654 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX11 (Maximum)

Pricing & Availability

Available

Domestic
International

Domestic Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

International Pricing for

Commercial & For-Profit

Not-For-Profit & Academic

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Prkdc<scid>, homozygous females and hemizygous males for Il2rg<tm1Wjl>, hemizigous or non-carrier for Tg(CSF1)3Sz

Related Products and Services

Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(CSF1)3Sz/SzJ	$2595.00
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(CSF1)3Sz/SzJ	$2595.00
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(CSF1)3Sz/SzJ	$3373.50
Frozen Mouse Embryo	NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl> Tg(CSF1)3Sz/SzJ	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Notice regarding distribution of this strain.

Use of MICE by companies or for-profit entities requires a no-fee JAX Leap License prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:NSG CSF1, NSG-CSF1

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Prkdcscid

Allele Symbol: Prkdcscid

Il2rgtm1Wjl

Allele Symbol: Il2rgtm1Wjl

Tg(CSF1)3Sz

Allele Symbol: Tg(CSF1)3Sz

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Il2rgtm1Wjl related

Genotype: Prkdcscid related

Mammalian Phenotype Terms by Genotype

References

Additional - Il2rgtm1Wjl related

Additional - Prkdcscid related

Additional - Tg(CSF1)3Sz related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Prkdc^scid

Allele Symbol: Prkdc^scid

Il2rg^tm1Wjl

Allele Symbol: Il2rg^tm1Wjl

Genotype: Il2rg^tm1Wjl related

Genotype: Prkdc^scid related

Additional - Il2rg^tm1Wjl related

Additional - Prkdc^scid related