These floxed mutant mice possess loxP sites flanking the first coding exon of the Snca gene. This strain may be useful for generating conditional mutations in applications related to neurodegenerative synucleinopathies, such as Parkinson's disease and multiple system atrophy.
Vladimir L Buchman, Cardiff University School of Biosciences
These Snca flox delta neo mice possess loxP sites on either side of the first coding exon (exon II) of the targeted gene. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the first coding exon deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele.
Mice that are homozygous for this allele are viable and fertile. The Donating Investigator reports that Western blot analysis of total protein extracts from several regions of the brain indicate that expression of the targeted mutation allele is similar to that observed in wildtype mice.
When crossed with transgenic mice expressing Cre recombinase under the control of the actin promoter, no expression of alpha-synuclein protein was detected in the resulting mice by Western blot analysis.
Mutations in human SNCA that result in aberrant polymerization and fibrillar aggregations are associated with neurodegenerative synucleinopathies, such as Parkinson's disease and multiple system atrophy.
A targeting vector containing a FRT site-flanked NEO cassette and a loxP site was inserted downstream of exon II (the first coding exon) of the targeted Snca gene, and another loxP site was inserted upstream of exon II. This construct was electroporated into C57BL/6N-Atm1Brd-derived JM8A3.N1 embryonic stem (ES) cells. The resulting chimeric animals were bred to C57BL/6J mice for 2 generations and then crossed to transgenic mice (on a congenic C57BL/6 genetic background) expressing FLP recombinase under the control of the human ACTB promoter. The FLP recombinase mediated excision of the FRT flanked NEO cassette and a 400 bp fragment encompassing a simple repeat region, which does not affect Snca locus expression. Snca flox delta neo mice that retained the floxed exon II were then bred to C57BL/6J mice for 2 generations to remove the FLP recombinase transgene. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
|Allele Name||targeted mutation 1.1, Vladimir L Buchman|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Sncatm1.1Vlb; targeted mutation 1.1, Vladimir L Buchman|
|Gene Symbol and Name||Snca, synuclein, alpha|
|Gene Synonym(s)||alphaSYN; alpha-synuclein; NACP; PARK4; PD1; PARK1; alpha-Syn|
|Strain of Origin||C57BL/6N-Atm1Brd|
|Molecular Note||A targeting vector containing a FRT site-flanked neo cassette and a loxP site was inserted downstream of exon 2 (the first coding exon) of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the SNCAflox delta neo mouse strain in a publication, please cite the originating article(s) and include JAX stock #025636 in your Materials and Methods section.
|Heterozygous or wildtype for Snca<tm1.1Vlb>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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