B6(SJL)-Cd180^{tm1c(KOMP)Wtsi}/KarpJ

Stock number: 024873
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These RP105 flox/flox mutant mice possess loxP sites flanking exon 3 of the Cd180 gene. This strain may be useful for generating conditional mutations in applications related to humoral immune responses.

Detailed description

The Cd180 gene encodes for the RP105 cell surface molecule that is a member of the Toll-like receptor family and is expressed on B cells, monocytes, macrophages and dendritic cells. These mice possess loxP sites on either side of exon 3 of the targeted gene. Exon 3 encodes the majority of the coding sequence for the Cd180 gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.

Development

This floxed allele of Cd180 is derived from D11 embryonic stem (ES) cells made by the knockout mouse project (KOMP), Wellcome Trust Sanger Institute. A vector containing an FRT site, lacZ coding sequence, loxP site, human beta-actin promoter controlling a neomycin resistance gene, SV40 polyA, a second FRT site and a second loxP site, was inserted upstream of exon 3. Another loxP site was inserted downstream of exon 3. This construct was electroporated into C57BL/6N-derived JM8.N4 embryonic stem (ES) cells. The chimeric mice were crossed to ACTB-FLPe mice (Stock No. 005703), to remove the selection cassette, leaving exon 3 of the gene flanked by loxP sites. The mice were crossed to C57BL/6NJ mice to remove the ACTB-FLPe transgene. Upon arrival at The Jackson Laboratory, mice were rederived with C57BL/6NJ (Stock No. 005304) at least once to establish the colony.

Allele

Symbol: Cd180^{tm1c(KOMP)Wtsi}
Name: targeted mutation 1c, Wellcome Trust Sanger Institute
MGI accession ID: MGI:6117108
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed)
Marker symbol: Cd180
Marker name: CD180 antigen
Marker synonyms: LY64; Ly78; RP105
Chromosome: 13
Strain of origin: C57BL/6N
Molecular note: The L1L2_Bact_P cassette was inserted at position 102840780 of Chromosome 13 upstream of exon 3 (Build GRCm39). The cassette contains an FRT site, lacZ coding sequence, loxP site, human beta-actin promoter controlling a neomycin resistance gene, SV40 polyA, a second FRT site and a second loxP site, was inserted upstream of exon 3. Another loxP site was inserted downstream of exon 3 at position 102843438. Flp-mediated recombination removed the FRT-flanked lacZ neo cassette, leaving exon 3 floxed. Exon 3 encodes the majority of the coding sequence for the gene. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml