Exon 3 of the Sec24c gene has been deleted in this Sec24c-Null mutant strain. These mice are useful in studies of embryonic development and endoplasmic reticulem (ER)-Golgi vesicular trafficking.
David Ginsburg, University of Michigan
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Sec24c | Sec24 related gene family, member C (S. cerevisiae) |
SEC24 proteins are responsible for selectively recruiting cargo proteins from the endoplasmic reticulum (ER) into COPII vesicles that mediate transport to the Golgi. Sec24c (Sec24 related gene family, member C (S. cerevisiae)) is one of four mammalian Sec24 paralogs which are highly conserved at the C-terminus and variable in the N-terminal region. Mice completely lacking SEC24C die around embryonic day 7.
Exon 3 of the Sec24c gene has been deleted in this null allele. Homozygotes die by embryonic day 7-8.5 for undetermined reasons. Heterozygotes can be intercrossed with Sec24c-FL floxed mice (see Stock No. 024867), then further crossed with Cre strains to develop tissue specific knockouts. Cre strains utilized by the donating laboratory include: P48-Cre (pancreas-specific), villin-Cre (intestinal-epithelial-specific (see Stock No. 004586)), albumin-Cre (hepatocyte-specific (see Stock No. 003574)),SM22-Cre (smooth-muscle-specific (see Stock No. 04746)),
and Meox2-Cre (ubiquitous expression beginning at embryonic day 5 (see Stock No. 003755)).
Embryonic stem (ES) cell clone EPD0241-2-A11 (EUCOMM ID: 32981) was created by the Wellcome Trust Sanger Institute as part of the Knockout Mouse Project (KOMP). The original gene trapped allele of Sec24c incorporated an FRT-En2 splice acceptor (SA)-IRES-lacZ-SV40 polyadenylation signal (pA)-loxP-PGK-Neo-SV40 polyadenylation signal (pA)-FRT-loxP sequence in intron 2 of the gene and an additional loxP site in intron 3 to create the Sec24c-GT (tm1a(EUCOMM)Wtsi) allele. C57BL/6N-derived JM8.N4 embryonic stem (ES) cells were used to create the mutation. Chimeras were crossed with B6(Cg)-Tyrc-2J/J (see Stock No. 000058) to achieve germline transmission. The lacZ and neomycin segments were excised with germline Flp recombinase (see Stock No. 005703), leaving exon 3 flanked by loxP sites. Then animals were crossed with a germline Cre strain (see Stock No. 003724) to delete exon 3. This strain was backcrossed to C57BL/6J for 3 generations by the donating laboratory. The Tyrc-2J allele was bred out of the line.
Allele Name | targeted mutation 1d, Wellcome Trust Sanger Institute |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Sec24c- |
Gene Symbol and Name | Sec24c, Sec24 related gene family, member C (S. cerevisiae) |
Gene Synonym(s) | |
Strain of Origin | C57BL/6N |
Chromosome | 14 |
General Note | Cell line EPD0241_2_A11 was successfully used to make chimeric mice. Germline transmission was accomplished. J:232065 |
Molecular Note | The L1L2_Pgk_P cassette was inserted at position 20679037 of Chromosome 14 upstream of exon 3. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 20679926. A null allele was created by flp and cre recombinase expression in mice, resulting in the deletion of the lacZ sequences, the neomycin selection cassette and exon 3. |
Heterozygotes are viable and fertile. Homozygous null mice die by embryonic day 7-8.5 for undetermined reasons.
When using the B6(Cg)-Sec24ctm1d(EUCOMM)Wtsi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #024868 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wildtype for Sec24c<tm1d(EUCOMM)Wtsi> |
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