These MIC transgenic mice express activated PyVT (Polyomavirus middle T antigen) and Cre recombinase under the control of a tetracycline-responsive promoter element (TRE or tetO) and are an effective tool for generating inducible, tissue-specific, targeted mutants to study polyomavirus middle T antigen (PyV mT)-mediated mammary tumorigenesis.
Dr. William J. Muller, McGill University
These MIC (TetO-Pyv mT-IRES-Cre) transgenic mice express activated PyVT (Polyomavirus middle T antigen) and IRES-Cre recombinase under the control of a tetO (tetracycline-responsive) promoter element. When these transgenic mice are bred with other transgenic mice expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue-specific transgene expression can be regulated with the tetracycline analog, doxycycline. Mice homozygous for the MIC are viable and fertile.
When crossed to a MMTV-rtTA strain, doxycycline induction results in development of multifocal mammary tumors within 16 days.
A transgenic vector was generated encoding the activated PyVT (Polyomavirus middle T antigen) and IRES-cre under control of a tetO (tetracycline-responsive element) promoter. The construct was microinjected into FVB/N fertilized oocytes. Founder line 1 was subsequently established. The mice were backcrossed to FVB/N for 10 generations by the donating lab (see SNP note below). Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize FVB/NJ oocytes (Stock No. 001800).
In 2019 a 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 8, was segregating with an unknown source.
|Allele Name||transgene insertion 1, William Muller|
|Allele Type||Transgenic (Recombinase-expressing, Inducible)|
|Allele Synonym(s)||MIC; TetO-Pyv mT-IRES-Cre|
|Gene Symbol and Name||Tg(tetO-PyVT,-cre)1Mul, transgene insertion 1, William Muller|
|Strain of Origin||FVB/N|
|Molecular Note||A transgene was generated where an activated PyVT (Polyomavirus middle T antigen) and cre recombinase (coupled by an internal ribosomal entry site (IRES)) are under control of the tet-responsive promoter (tetO).|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the MIC, TetO-Pyv mT-IRES-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #024864 in your Materials and Methods section.