These p600flox/flox mutant mice possess loxP sites flanking exon 1 of the ubiquitin protein ligase E3 component n-recognin 4 (Ubr4) gene. P600 is involved in cytoskeletal organization, membrane morphogenesis, integrin-mediated survival signaling, calcium-calmodulin signaling, and the N-end rule pathway of ubiquitin-proteasome-mediated proteolysis. Embryonic p600 is essential for liver, cardiac, and brain development. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.
For example, when bred to B6.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 008454) expressing Cre-recombinase under the Sox2 promoter, resulting mice die by E14.5 due to severe cardiac problems including ventricular septal defects and thin ventricular walls. Brain of the knockout mice displays thinner cortical plate with significant less Tuj1-positive neurons. Starting from E13.5, the animals display enlarged ventricles. The orientation of the mitotic spindle in cortical neural progenitors is randomized in E12.5 animals.
A targeting vector was designed to insert a loxP site followed by a frt-flanked neomycin resistance (neo) cassette upstream of exon 1, and a second loxP site downstream of exon 1 of the ubiquitin protein ligase E3 component n-recognin 4 (Ubr4) gene. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred with C57BL/6NCrl females. Offspring were bred with B6;SJL-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 003800) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. The donating investigator reported that these p600flox/flox mice were bred to C57BL/6NCrl mice for at least 15 generations (See SNP note below). Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 5 markers on three choromosomes were segregating, suggesting an incomplete backcross.
|Allele Name||targeted mutation 1.2, Yoshihiro Nakatani|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ubr4, ubiquitin protein ligase E3 component n-recognin 4|
|Gene Synonym(s)||1810009A16Rik; 1810009A16Rik; A930005E13Rik; A930005E13Rik; D930005K06Rik; D930005K06Rik; Gm1032; LOC381562; N28143; RBAF600; RIKEN cDNA 1810009A16 gene; RIKEN cDNA A930005E13 gene; RIKEN cDNA D930005K06 gene; Rbaf600; ZUBR1; Zubr1; Zubr1; expressed sequence N28143; gene model 1032, (NCBI); mKIAA0462; p600; zinc finger, UBR1 type 1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 1. An additional loxP site was inserted downstream of exon 1. Flp-mediated recombination removed the neomycin resistance cassette and left exon 1 floxed.|
When maintaining a live colony, homozygous mice may be bred together.
When using the p600 flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #024844 in your Materials and Methods section.
|Heterozygous for Ubr4<tm1.2Nkt>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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