These Tgm2t/t floxed mice may be useful for studying the regulation of cell adhesion and protein aggregation.
Robert M Graham, Victor Chang Cardiac Research Institute
These Tgm2t/t floxed mice possess loxP sites flanking exons 6-8 of the transglutaminase 2, C polypeptide (Tgm2) gene. The donating investigator states that the remaining frt-flanked neo cassette conveys no abnormalities. Tgm2 is a calcium-dependent enzyme that catalyzes the crosslinking of proteins. TGM2 mediates signaling by various G-protein coupled receptors and has been found to be involved in apoptosis, fibronectin stabilization, cataract development, neurodegeneration, and wound healing. TGM2 has also been cited as an autoantigen involved in celiac disease and the onset of Type 2 diabetes mellitus (T2DM). Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 6-8 deleted in the cre-expressing tissues.
For example, when bred to mice carrying the Tg(CMV-cre)1Cgn allele ( see Stock No. 006054), mice exhibit normal glucose tolerance, insulin sensitivity and glucose stimulated insulin secretion on a standard or high fat diet. Fibroblasts from these mice exhibit decreased adhesion. When these TGM2-deficient mice are bred to B6CBA-Tg(HDexon1)62Gpb/1J mice (Stock No. 002810), resulting offspring contain more huntingtin protein aggregates within the cerebral cortex and striatum, with no difference in the onset of motor dysfunction and the mean lifespan, compared to Tg(HDexon1)62Gpb transgenic mice.
A targeting vector was designed to insert a loxP site and a frt-flanked neomycin resistance (neo) cassette upstream of exon 6, and a second loxP site downstream of exon 8 of transglutaminase 2, C polypeptide (Tgm2) gene. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice. These mice were bred to C57BL/6J mice for at least 12 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Robert M Graham|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Tgm2, transglutaminase 2, C polypeptide|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||LoxP sites that flank exons 6-8 were inserted by homologous recombination.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Tgm2t mouse strain in a publication, please cite the originating article(s) and include JAX stock #024694 in your Materials and Methods section.