These Tgm2t/t floxed mice may be useful for studying the regulation of cell adhesion and protein aggregation.
Robert M Graham, Victor Chang Cardiac Research Institute
Genetic Background | Generation |
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N12+pN2
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Tgm2 | transglutaminase 2, C polypeptide |
These Tgm2t/t floxed mice possess loxP sites flanking exons 6-8 of the transglutaminase 2, C polypeptide (Tgm2) gene. The donating investigator states that the remaining frt-flanked neo cassette conveys no abnormalities. Tgm2 is a calcium-dependent enzyme that catalyzes the crosslinking of proteins. TGM2 mediates signaling by various G-protein coupled receptors and has been found to be involved in apoptosis, fibronectin stabilization, cataract development, neurodegeneration, and wound healing. TGM2 has also been cited as an autoantigen involved in celiac disease and the onset of Type 2 diabetes mellitus (T2DM). Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 6-8 deleted in the cre-expressing tissues.
For example, when bred to mice carrying the Tg(CMV-cre)1Cgn allele ( see Stock No. 006054), mice exhibit normal glucose tolerance, insulin sensitivity and glucose stimulated insulin secretion on a standard or high fat diet. Fibroblasts from these mice exhibit decreased adhesion. When these TGM2-deficient mice are bred to B6CBA-Tg(HDexon1)62Gpb/1J mice (Stock No. 002810), resulting offspring contain more huntingtin protein aggregates within the cerebral cortex and striatum, with no difference in the onset of motor dysfunction and the mean lifespan, compared to Tg(HDexon1)62Gpb transgenic mice.
A targeting vector was designed to insert a loxP site and a frt-flanked neomycin resistance (neo) cassette upstream of exon 6, and a second loxP site downstream of exon 8 of transglutaminase 2, C polypeptide (Tgm2) gene. The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice. These mice were bred to C57BL/6J mice for at least 12 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Robert M Graham |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Tgm2t |
Gene Symbol and Name | Tgm2, transglutaminase 2, C polypeptide |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 2 |
Molecular Note | LoxP sites that flank exons 6-8 were inserted by homologous recombination. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Tgm2t mouse strain in a publication, please cite the originating article(s) and include JAX stock #024694 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tgm2<tm1Rmgr> |
Frozen Mouse Embryo | B6.129S1-Tgm2<tm1Rmgr>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Tgm2<tm1Rmgr>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Tgm2<tm1Rmgr>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Tgm2<tm1Rmgr>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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