Trpc1 (transient receptor potential cation channel, c1) knockout mice exhibit an altered allergic immune response and nervous system electrophysiology.
Lutz Birnbaumer, National Institutes of Health
Trpc1 (transient receptor potential cation channel, c1) encodes a mechanosensitive and store-operated cation channel. Homozygous knockout mice exhibit reduced allergan-induced pulmonary leukocyte infiltration and reduced T helper 2 responses. Splenocytes exposed to allergan have impaired proliferation and IL2 production. In addition, members of the TRPC family are associated with epileptogenesis and neuronal excitotoxicity. Trpc1 knockout mice have reduced epileptiform burst firing induced by m GluR agonists. Plateau amplitudes and spike numbers per burst are reduced.
This strain may be useful for studying regulation of allergan-induced lung hyperresponsiveness and seizure physiology.
A targeting vector containing a neomycin resistance cassette was used to disrupt the terminal part of exon 8. In the protein this results in the loss of transmembrane segments 4 and 5 and a small part of the pore domain. The construct was electroporated into 129S/SvEv-derived EK.CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice for one generation. The donating investigator reported that a homozygous colony was established and maintained by interbreeding (see SNP note). Upon arrival, mice were bred to 129S1/SvImJ for 1 generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Five of the 27 markers throughout the genome were segregating from an unknown source.
|Allele Name||targeted mutation 1, Lutz Birnbaumer|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Trpc1, transient receptor potential cation channel, subfamily C, member 1|
|Strain of Origin||129S/SvEv-Gpi1c|
|Molecular Note||An EcoRI site in exon 8 was converted to a stop codon and a PGK-neo selection cassette replaced the terminal part of exon 8 along with intron 8. This resulted in loss of the coding region for amino acids 584 - 670 encoding transmembrane segments 4 and 5 and a small part of the putative pore domain. RT-PCR analysis detected a shorter transcript in which all of exon 8 was skipped resulting in a frameshift and stop codon at position 2 of exon 9. Western blot analysis confirmed absence of expression in the submandibular glands of homozygous mice.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the Trpc1- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37347 in your Materials and Methods section.