Gnao1 (guanine nucleotide binding protein, alpha O) knockout mice exhibit decreased survival, tremors, hyperalgesia, hyperactivity and circling behaviors.
Lutz Birnbaumer, National Institutes of Health
Gnao1 (guanine nucleotide binding protein, alpha O) or Go1alpha encodes the alpha subunit of a signal-transducing heterotrimeric Go protein. Go is the most abundant G protein found in neurons. Mice homozygous for this targeted knockout have a decreased survival, 50% die at 7 weeks of age. In addition, there is evidence of embryonic lethality. Mice exhibit a generalized tremor, impaired motor control, an increased sensitivity to pain (hyperalgesia), hyperactivity, and circling behavior. Muscarinic inhibition of Ca2+ current is impaired in these mice. This strain may be useful for studying the role of Go protein in motor control, motor behavior, pain perception and Ca2+ regulation.
A targeting vector containing a neomycin resistance cassette was used to disrupt exon 6, an exon common to both splice variants and preventing synthesis of all forms of the gene. The construct was electroporated into 129S7/SvEvBrd-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice for an unknown number of generations. Upon arrival, mice were bred to 129S1/SvImJ for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Lutz Birnbaumer|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Gnao1.1tm1LBi; Go1alpha|
|Gene Symbol and Name||Gnao1, guanine nucleotide binding protein, alpha O|
|Strain of Origin||129S7/SvEvBrd-Hprtb-m2|
|Molecular Note||The neo cassette was inserted into exon 6. No protein was detected in homogenates from homozygous targeted mice by ADP-ribosylation with pertussis toxin.|
While maintaining a live colony, these mice are bred as heterozygotes. Mice homozygous for the mutation do not breed well and often die before 7 weeks of age.
When using the 129S-Gnao1tm1Lbi/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #37339 in your Materials and Methods section.