A frameshift mutation was placed into exon 1 and a neomycin resistance (neo) cassette replaces part of exon 3 of the interleukin 3 (Il3) gene, abolishing gene expression in this strain. IL-3 is a cytokine that promotes the survival, proliferation, and differentiation of hematopoietic progenitors and mature hematopoietic cells. Homozygous mice are viable and fertile. Bone marrow derived mast cells (BMMC) from IL-3 deficient mice exhibit decreased survival when induced with IgE. Expression of anti-apoptotic proteins, Bcl-xL and Bcl-2 are decreased after IgE-induction. When colony stimulating factor 2 receptor, beta (Csf2rd) is knocked out of these mice, double mutants exhibit a lack of eosinophilic response to parasites, and show a pulmonary alveolar proteinosis-like disease.
A targeting vector was designed insert a frameshift mutation at the HindIII site in exon 1 and to replace a 463bp fragment exon 3 of the interleukin 3 (Il3) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred to C57BL/6JNimr females. These mice were bred to C57BL/10ScSnJNimr mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/10ScSnJ (Stock No. 000476) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Victor L J Tybulewicz|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Il3, interleukin 3|
|Strain of Origin||129S2/SvPas|
|Molecular Note||As a result of a frameshift mutation in exon 1 and the deletion of exon 2, this allele lacks functional protein.|
When maintaining a live colony, homozygous mice may be bred together.
When using the B10.129S2(B6)-Il3tm1Tyb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023816 in your Materials and Methods section.