These Dact1flox mice may be useful for studying embryonic and neuronal development.
Benjamin N R Cheyette, UCSF School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Dact1 | dishevelled-binding antagonist of beta-catenin 1 |
These Dact1flox mice possess loxP sites flanking exon 2 of the dapper homolog 1, antagonist of beta-catenin (Dact1) gene. Dact1 encodes an intracellual protein required for morphogenesis at the primitive streak, and for dendrite, spine, and excitatory synapse development in the mouse forebrain. Through interactions with the Dishevelled family of proteins, DACT1 modulates the transcriptional activation of target genes of the Wnt/beta-catenin signaling pathway as well as beta-catenin-independent branches of Wnt signaling. Homozygotes are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues.
For example, when bred to B6.129S2-Emx1tm1(cre)Krj/J mice
(Stock No. 005628) expressing Cre recombinase in the neurons of the neocortex and hippocampus, neurons from double mutant Dact1-deficient mice exhibit reduced dendritic spine density, spine length, and spine head width.
A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette, followed by a loxP site, upstream of exon 2, and a second loxP site downstream of exon 2 of the dapper homolog 1, antagonist of beta-catenin (Dact1) gene. The construct was electroporated into 129Sv embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene. These mice were bred to C57BL/6 mice for at least 10 generations. Subsequenty, they have been bred to CAG-CAT-EGFP transgenic mice on a CD-1 background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony. The CAG-CAT-EGFP transgene was removed from the colony.
Allele Name | targeted mutation 1.2, Benjamin N R Cheyette |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Dact1flox |
Gene Symbol and Name | Dact1, dishevelled-binding antagonist of beta-catenin 1 |
Gene Synonym(s) | |
Strain of Origin | 129 |
Chromosome | 12 |
Molecular Note | A targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette, followed by a loxP site, upstream of exon 2, and a second loxP site downstream of exon 2. Flp-mediated recombination removed the neo cassette leaving exon 2 floxed. |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Dact1tm1.2Bnrc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023715 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Dact1<tm1.2Bnrc> |
Frozen Mouse Embryo | STOCK Dact1<tm1.2Bnrc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Dact1<tm1.2Bnrc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Dact1<tm1.2Bnrc>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Dact1<tm1.2Bnrc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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