These Vspb-/- mice may be useful for studying risk factors for motor neuron diseases such as amyotrophic lateral sclerosis (ALS).
Luc Dupuis, Universite de Strasbourg
These Vspb-/- mutant mice lack exon 3 of the vesicle-associated membrane protein, associated protein B and C (Vapb) gene, abolishing gene expression. Vspb encodes a membrane protein involved in vesicle trafficking, membrane fusion, protein complex assembly, and cell motility. Mutations and loss of VAPB function have been shown to contribute to motor deficits associated with amyotrophic lateral sclerosis (ALS) pathogenesis. Homozygotes are viable and fertile. Adult Vapb KO mice develop mild, late onset central nervous system defects including mild motor deficits after 18 months of age and mild tremors in open field tests. These mice do not exhibit defects in neuromuscular junction or muscle strength, and there is no apparent muscle denervation.
A targeting vector was designed to insert a loxP site upstream of exon 3, and a second loxP site followed by a frt-flanked PGK-neo cassette just downstream of exon 3 of the vesicle-associated membrane protein, associated protein B and C (Vapb) gene. The construct was electroporated into 129S2/SvPas-derived P1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to mice expressing Frt-recombinase to remove the neo cassette. Offspring were crossed to remove the Frt-containing transgene and progeny were bred to CMV-cre transgenic mice to remove the floxed-sequence. Subsequently, these Vspb-/- mice were bred to C57BL/6NTac mice once and were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for two generations to establish the colony.
|Allele Name||targeted mutation 1.2, Mouse Clinical Institute|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Vapbtm1.2Ics; targeted mutation 1.2, Mouse Clinical Institute|
|Gene Symbol and Name||Vapb, vesicle-associated membrane protein, associated protein B and C|
|Gene Synonym(s)||ALS8; DNA segment, Chr 2, Abbott 2 expressed; R75548; expressed sequence AI225786; expressed sequence AI848259; expressed sequence R75548; VAP-B; VAP33b; VAMP-B; AI225786; D2Abb2e; VAMP-associated protein 33b; expressed sequence AI840687; AI848259; AI840687; D2Abb2e|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Cre-mediated recombination removed a floxed neomycin resistance cassette. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette. Western blot anlaysis confirmed the absence of protein expression in the cerebral cortex.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Vapb- mouse strain in a publication, please cite the originating article(s) and include JAX stock #023592 in your Materials and Methods section.
|Heterozygous or Wildtype for|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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