These Cd48 knock-out mice, on a congenic C57BL/6 background, develop auto-antibodies and glomerulonephritis. They are suitable for use in applications related to the study of T-cell tolerance and systemic lupus erythematosus.
Elahna Paul, Massachusetts General Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Cd48 | CD48 antigen |
The Cd48 gene, also know as Slamf2 (signaling lymphocytic activation molecule 2), encodes for a lymphocyte cell surface antigen that binds CD2 and CD244 and is involved in regulation of T cell activation.
These mice carry a targeted mutation for Cd48, in which a NEO cassette has replaced the exon encoding the immunoglobulin-V-like domain (the binding domain for CD2). Mice that are homozygous for the targeted mutation are viable and fertile. No cell surface CD48 protein is detected by flow cytometric analysis of thymocytes and splenocytes from homozygous animals. Female homozygotes develop auto-antibodies by 3 months, and glomerulonephritis by 6 months of age.
Homozygotes exhibit increased numbers of short term hematopoietic stem cells (HSC) and multipotent progenitor cells, while decreased numbers of myeloid progenitor cells. HSCs from homozygotes have impaired short term engraftment in transplantation studies, as well as defective proliferation (increased quiescence). Cytokine and interferon gamma levels in the bone marrow of homozygotes are significantly reduced. Homozygotes on the congenic C57BL/6 background have a shortened lifespan (most die by 80 weeks of age) compared to wildtype controls. By approximately 16 weeks of age, most homozygotes have developed tumors (predominantly lymphomas).
A targeting vector designed by Dr. Arlene Sharpe (Harvard Medical School) containing a NEO cassette was used to disrupt the exon encoding the immunoglobulin-V-like domain. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into C57BL/6 blastocysts.
The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6J for 12 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. Upon arrival at The Jackson Laboratory Repository, the mice were crossed to C57BL/6J inbred mice (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, Hans Reiser |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | CD48- |
Gene Symbol and Name | Cd48, CD48 antigen |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 1 |
Molecular Note | A neomycin selection cassette replaced the exon encoding the immunoglobulin-V-like domain, which forms a binding site for the Cd2 antigen. Thymocytes and splenocytes isolated from homozygous mutant mice were found to lack surface expression of the endogenous protien. |
Although homozygous mice are fertile, The Jackson Laboratory Repository maintains its live colony by breeding heterozygous mice together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the Slamf2-/- mouse strain in a publication, please cite the originating article(s) and include JAX stock #023536 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cd48<tm1Rsr> |
Frozen Mouse Embryo | B6.129S4-Cd48<tm1Rsr>/EpaulJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Cd48<tm1Rsr>/EpaulJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Cd48<tm1Rsr>/EpaulJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Cd48<tm1Rsr>/EpaulJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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