cellular phenotype

• decreased T cell proliferation
  • homozygotes exhibit impaired T cell proliferation in response to stimulation with lectins, anti-CD3 antibodies, and alloantigens; however, no proliferative defects are detected when mutant splenocytes are activated with LPS (a B cell mitogen)
  (MGI Ref ID J:53718)
  • ion of calcium ionophore and PMA remain unaffected
  (MGI Ref ID J:53718)
  • the proliferative responses of mutant splenocytes to Con A and anti-CD3 mAb are reduced by ~20%-50%, while responses to phytohemagglutinin (PHA) are almost completely abolished
  (MGI Ref ID J:53718)
  • in addition, the proliferative responses of highly-purified mutant CD4⁺ T cells to TCR stimulation with either anti-CD3 mAb and phorbol 12-myristate 13-acetate (PMA) or with Con A and PMA are abrogated, whereas responses to a mitogenic combination of calcium ionophore and PMA remain unaffected
  (MGI Ref ID J:53718)

immune system phenotype

• decreased T cell proliferation
  • homozygotes exhibit impaired T cell proliferation in response to stimulation with lectins, anti-CD3 antibodies, and alloantigens; however, no proliferative defects are detected when mutant splenocytes are activated with LPS (a B cell mitogen)
  (MGI Ref ID J:53718)
  • the proliferative responses of mutant splenocytes to Con A and anti-CD3 mAb are reduced by ~20%-50%, while responses to phytohemagglutinin (PHA) are almost completely abolished
  (MGI Ref ID J:53718)
  • ion of calcium ionophore and PMA remain unaffected
  (MGI Ref ID J:53718)
  • in addition, the proliferative responses of highly-purified mutant CD4⁺ T cells to TCR stimulation with either anti-CD3 mAb and phorbol 12-myristate 13-acetate (PMA) or with Con A and PMA are abrogated, whereas responses to a mitogenic combination of calcium ionophore and PMA remain unaffected
  (MGI Ref ID J:53718)

• increased CD4-positive, alpha beta T cell number
  • Normal - however, overall lymphoid development is grossly normal, as determined by dual color immunofluorescence and flow cytometry
  (MGI Ref ID J:53718)
  • homozygotes display a slight increase in the fraction of CD4⁺ CD8^- T cells in thymus and spleen
  (MGI Ref ID J:53718)

• abnormal T cell activation
  • homozygotes display a marked defect in CD4⁺ T cell activation mediated through the T cell receptor/CD3 complex
  (MGI Ref ID J:53718)

hematopoietic system phenotype

• decreased T cell proliferation
  • homozygotes exhibit impaired T cell proliferation in response to stimulation with lectins, anti-CD3 antibodies, and alloantigens; however, no proliferative defects are detected when mutant splenocytes are activated with LPS (a B cell mitogen)
  (MGI Ref ID J:53718)
  • ion of calcium ionophore and PMA remain unaffected
  (MGI Ref ID J:53718)
  • the proliferative responses of mutant splenocytes to Con A and anti-CD3 mAb are reduced by ~20%-50%, while responses to phytohemagglutinin (PHA) are almost completely abolished
  (MGI Ref ID J:53718)
  • in addition, the proliferative responses of highly-purified mutant CD4⁺ T cells to TCR stimulation with either anti-CD3 mAb and phorbol 12-myristate 13-acetate (PMA) or with Con A and PMA are abrogated, whereas responses to a mitogenic combination of calcium ionophore and PMA remain unaffected
  (MGI Ref ID J:53718)

• increased CD4-positive, alpha beta T cell number
  • homozygotes display a slight increase in the fraction of CD4⁺ CD8^- T cells in thymus and spleen
  (MGI Ref ID J:53718)
  • Normal - however, overall lymphoid development is grossly normal, as determined by dual color immunofluorescence and flow cytometry
  (MGI Ref ID J:53718)

• abnormal T cell activation
  • homozygotes display a marked defect in CD4⁺ T cell activation mediated through the T cell receptor/CD3 complex
  (MGI Ref ID J:53718)