The targeted Orai1 gene encodes a pore subunit of the store-operated calcium channel, CRAC (calcium release-activated calcium) channel. Mutations in this gene have been associated with primary immunodeficiency-9 and tubular aggregate myopathy-2. These knock-out mice carry an allele of Orai1 in which exon 1 has been excised. 
No gene product (mRNA) is detected by RT-PCR analysis of CD4+ T cells and CD19+ B cells from homozygotes. Fewer than expected homozygotes on the C57BL/6 background are born, and homozygotes are perinatal lethal, with all homozygous pups dying by 1.5 days after birth.
MEFs isolated from homozygotes exhibit significantly impaired store-operated Ca2+ influx.

Use of MICE by companies or for-profit entities requires a license prior to shipping.

This Orai1 knock-out strain is useful in studies of regulation of cytosolic and endoplasmic reticulum calcium levels, store-operated calcium channels and T cell and B cell function.

A minimum of two mice must be ordered. Additional mice will be charged on a per-mouse basis. Click the “Details” button to learn more.

Our minimum guarantee for cryorecovery orders is 2 carriers. To ensure that your colony can be rapidly expanded upon arrival, additional carriers may be added to your order.