The p48-cre knock-in/knock-out allele has a Cre recombinase gene inserted into the first coding exon of the Ptf1a gene; both abolishing endogenous Ptf1a gene function and placing cre expression under the control of the endogenous Ptf1a promoter/enhancer elements. Cre recombinase activity occurs in the developing pancreas, neural tube, cerebellum and retina. These mice may be useful for cre-lox studies of pancreatic development.
Roland M. Schmid, Technical University of Munich
Henrik Einwaechter, Technical University of Munich
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Ptf1a | pancreas specific transcription factor, 1a |
The Ptf1a gene encodes a transcription factor involved in pancreatic organogenesis. This p48-cre strain expresses Cre recombinase from the endogenous Ptf1a locus and results in a null allele. Cre recombinase sequence was inserted at the start codon of the gene. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in embryonic mice, at E10.5, in the pancreas (specifically the prepancreatic endoderm and progenitor and exocrine cells), neural tube, cerebellum and retina. Germline expression is also described below. Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygotes are not viable, dying within 3 hours of birth.
Kabacaoglu et al. 2020 bioRxiv reports that p48-cre mice express Cre in male germline. The recombination event was observed in multiple floxed alleles, while the frequency varied depending on the target gene. Additionally, Cre allele transmission into progeny was not necessary for the germline recombination. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding p48-cre females with floxed males.
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Ptf1atm1(cre)Hnak). This same information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.
A targeting vector containing cre coding sequence and a FRT site flanked NEO cassette was used to disrupt part of exon 1 of the targeted Ptf1a gene. The endogenous Ptf1a promoter drives expression of the Cre recombinase coding sequence. The construct was electroporated into unspecified embryonic stem (ES) cells which were transiently transfected with a FLP recombinase plasmid to remove the selection cassette. ES cells that had successfully undergone FLP-mediated recombination and no longer retained the NEO cassette were injected into blastocysts.
The resulting chimeric animals were tested for germline transmission. The strain was maintained on a mixed B6;129 background but could contain contributions from other genetic backgrounds.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombination occurs in embryonic mice, at E10.5, in the pancreas (specifically the prepancreatic endoderm and progenitor and exocrine cells), neural tube, cerebellum and retina. |
Allele Name | targeted mutation 1, Hassan Nakhai |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | p48-cre; Ptf1aCre(ex1); Ptf1atm1(cre)Nak; Ptf1a-cre(ex1) |
Gene Symbol and Name | Ptf1a, pancreas specific transcription factor, 1a |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombination occurs in embryonic mice, at E10.5, in the pancreas (specifically the prepancreatic endoderm and progenitor and exocrine cells), neural tube, cerebellum and retina. |
Strain of Origin | Not Specified |
Chromosome | 2 |
Molecular Note | Cre recombinase replaced part of exon 1. A neomycin resistance gene included in the vector was subsequently removed via transient FLP expression. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes are not viable, dying within 3 hours of birth.
Kabacaoglu et al. 2020 bioRxiv reports that p48-cre mice express Cre in male germline. The recombination event was observed in multiple floxed alleles, while the frequency varied depending on the target gene. Additionally, Cre allele transmission into progeny was not necessary for the germline recombination. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding p48-cre females with floxed males.
When using the p48-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #023329 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ptf1a<tm1(cre)Hnak> |
Frozen Mouse Embryo | STOCK Ptf1a<tm1(cre)Hnak>/RschJ | $2595.00 |
Frozen Mouse Embryo | STOCK Ptf1a<tm1(cre)Hnak>/RschJ | $2595.00 |
Frozen Mouse Embryo | STOCK Ptf1a<tm1(cre)Hnak>/RschJ | $3373.50 |
Frozen Mouse Embryo | STOCK Ptf1a<tm1(cre)Hnak>/RschJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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