The Ptf1a gene encodes a transcription factor involved in pancreatic organogenesis. This p48-cre strain expresses Cre recombinase from the endogenous Ptf1a locus and results in a null allele. Cre recombinase sequence was inserted at the start codon of the gene. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in embryonic mice, at E10.5, in the pancreas (specifically the prepancreatic endoderm and progenitor and exocrine cells), neural tube, cerebellum and retina. Germline expression is also described below. Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygotes are not viable, dying within 3 hours of birth. <a href="https://pubmed.ncbi.nlm.nih.gov/34175279/">Kabacaoglu et al. 2021 Gastroenterology 161:1695</a> reports that p48-cre mice express Cre in male germline. The recombination event was observed in multiple floxed alleles, while the frequency varied depending on the target gene. Additionally, Cre allele transmission into progeny was not necessary for the germline recombination. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding p48-cre females with floxed males. If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (<a href="http://www.informatics.jax.org/allele/MGI:3701996?recomRibbon=open">Ptf1atm1(cre)Hnak</a>). This same information may also be found searching the <a href="http://www.informatics.jax.org/home/recombinase">MGI Recombinase Activity</a> and <a href="http://www.informatics.jax.org/gxd/recombinasegrid/MGI:1328312">MGI Gene Expression + Recombinase Activity Comparison Matrix</a>.

The p48-cre knock-in/knock-out allele has a Cre recombinase gene inserted into the first coding exon of the Ptf1a gene; both abolishing endogenous Ptf1a gene function and placing cre expression under the control of the endogenous Ptf1a promoter/enhancer elements. Cre recombinase activity occurs in the developing pancreas, neural tube, cerebellum, retina and male germline. These mice may be useful for Cre-lox studies of pancreatic development.

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