Overview

Also Known As:p48-cre

The p48-cre knock-in/knock-out allele has a Cre recombinase gene inserted into the first coding exon of the Ptf1a gene; both abolishing endogenous Ptf1a gene function and placing cre expression under the control of the endogenous Ptf1a promoter/enhancer elements. Cre recombinase activity occurs in the developing pancreas, neural tube, cerebellum and retina. These mice may be useful for cre-lox studies of pancreatic development.

Donating Investigator

Roland M. Schmid, Technical University of Munich

Henrik Einwaechter, Technical University of Munich

Genetic overview

Genetic Background	Generation

Ptf1a^tm1(cre)Hnak

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Ptf1a	pancreas specific transcription factor, 1a

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Research Applications

Research Tools
Developmental Biology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Ptf1a gene encodes a transcription factor involved in pancreatic organogenesis. This p48-cre strain expresses Cre recombinase from the endogenous Ptf1a locus and results in a null allele. Cre recombinase sequence was inserted at the start codon of the gene. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in embryonic mice, at E10.5, in the pancreas (specifically the prepancreatic endoderm and progenitor and exocrine cells), neural tube, cerebellum and retina. Germline expression is also described below. Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygotes are not viable, dying within 3 hours of birth.

Kabacaoglu et al. 2020 bioRxiv reports that p48-cre mice express Cre in male germline. The recombination event was observed in multiple floxed alleles, while the frequency varied depending on the target gene. Additionally, Cre allele transmission into progeny was not necessary for the germline recombination. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding p48-cre females with floxed males.
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Ptf1a^tm1(cre)Hnak). This same information may also be found searching the MGI Recombinase Activity and MGI Gene Expression + Recombinase Activity Comparison Matrix.

Development

A targeting vector containing cre coding sequence and a FRT site flanked NEO cassette was used to disrupt part of exon 1 of the targeted Ptf1a gene. The endogenous Ptf1a promoter drives expression of the Cre recombinase coding sequence. The construct was electroporated into unspecified embryonic stem (ES) cells which were transiently transfected with a FLP recombinase plasmid to remove the selection cassette. ES cells that had successfully undergone FLP-mediated recombination and no longer retained the NEO cassette were injected into blastocysts.
The resulting chimeric animals were tested for germline transmission. The strain was maintained on a mixed B6;129 background but could contain contributions from other genetic backgrounds.
Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombination occurs in embryonic mice, at E10.5, in the pancreas (specifically the prepancreatic endoderm and progenitor and exocrine cells), neural tube, cerebellum and retina.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Nakhai H; Sel S; Favor J; Mendoza-Torres L; Paulsen F; Duncker GI; Schmid RM. 2007. Ptf1a is essential for the differentiation of GABAergic and glycinergic amacrine cells and horizontal cells in the mouse retina. Development 134(6):1151-60PubMed: 17301087 MGI: J:119184

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Genetics

Ptf1a^tm1(cre)Hnak

Allele Symbol: Ptf1a^tm1(cre)Hnak

Allele Name	targeted mutation 1, Hassan Nakhai
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	p48-cre; Ptf1a^Cre(ex1); Ptf1a^tm1(cre)Nak; Ptf1a-cre(ex1)
Gene Symbol and Name	Ptf1a, pancreas specific transcription factor, 1a
Gene Synonym(s)
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombination occurs in embryonic mice, at E10.5, in the pancreas (specifically the prepancreatic endoderm and progenitor and exocrine cells), neural tube, cerebellum and retina.
Strain of Origin	Not Specified
Chromosome	2
Molecular Note	Cre recombinase replaced part of exon 1. A neomycin resistance gene included in the vector was subsequently removed via transient FLP expression.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Developmental Biology Research
  - Cre-lox System
- Metabolism Research
Developmental Biology Research

Mammalian Phenotype Terms by Genotype

References

Nakhai H; Sel S; Favor J; Mendoza-Torres L; Paulsen F; Duncker GI; Schmid RM. 2007. Ptf1a is essential for the differentiation of GABAergic and glycinergic amacrine cells and horizontal cells in the mouse retina. Development 134(6):1151-60PubMed: 17301087 MGI: J:119184

Additional - Ptf1a^tm1(cre)Hnak related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Generic Cre Probe
- Standard PCR:Ptf1a Alt 1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice (Stock No. 000664). Homozygotes are not viable, dying within 3 hours of birth.

Kabacaoglu et al. 2020 bioRxiv reports that p48-cre mice express Cre in male germline. The recombination event was observed in multiple floxed alleles, while the frequency varied depending on the target gene. Additionally, Cre allele transmission into progeny was not necessary for the germline recombination. As such, for Cre-lox experiments and to avoid/minimize germline deletion of the floxed allele, researchers may consider breeding p48-cre females with floxed males.
- Additional Breeding and Husbandry Support
Mating System
- Wild-type x Heterozygote
- Heterozygote x Wild-type
Citation
When using the p48-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #023329 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	Heterozygous or wildtype for Ptf1a<tm1(cre)Hnak>

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Frozen Mouse Embryo	STOCK Ptf1a<tm1(cre)Hnak>/RschJ	$2595.00
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Frozen Mouse Embryo	STOCK Ptf1a<tm1(cre)Hnak>/RschJ	$3373.50
Frozen Mouse Embryo	STOCK Ptf1a<tm1(cre)Hnak>/RschJ	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:p48-cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Ptf1atm1(cre)Hnak

Allele Symbol: Ptf1atm1(cre)Hnak

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Ptf1atm1(cre)Hnak related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Ptf1a^tm1(cre)Hnak

Allele Symbol: Ptf1a^tm1(cre)Hnak

Additional - Ptf1a^tm1(cre)Hnak related