These floxed mutant mice possess loxP sites flanking exons 5-12 of the Ilk gene. This strain may be useful for generating conditional mutations in applications related to examining the role played by Ilk in cellular development and homeostasis.
Rene St-Arnaud, McGill University
These ILKfl/fl mice possess loxP sites flanking exons 5-12 of the integrin linked kinase (Ilk) gene. ILK is a transmembrane serine/threonine protein kinase involved in gene expression, cell proliferation, migration, adhesion, and survival. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5-12 deleted in cre-expressing tissues.
For example, when crossed to B6.Cg-Tg(ACTA1-cre)79Jme/J mice (Stock No. 006149), expressing Cre recombinase in striated muscle, this mutant mouse strain develops widespread progressive muscular dystrophy.
When crossed to STOCK Tg(KRT14-cre)1Amc/J mice (Stock No. 004782), expressing Cre recombinase in skin, this mutant mouse strain dies perinatally and exhibits skin blistering and severe defects in hair follicle morphogenesis.
When crossed to B6.Cg-Tg(NPHS2-cre)295Lbh/J mice (Stock No. 008205), expressing Cre recombinase in kidney podocytes, this mutant mouse strain develops albuminuria, glomerulosclerosis, and kidney failure, leading to death beginning at 10 wk of age.
When crossed to B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J mice (Stock No. 005249), expressing RU-486 induced Cre recombinase in epithelial and hair follicle stem cells, this mutant mouse strain exhibits impaired cutaneous wound healing and decreased wound closure rates.
A targeting vector was designed to insert a single loxP site upstream of exon 5, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 12 of the integrin linked kinase (Ilk) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pBS185 cre expression plasmid to delete the neo cassette. Resulting ES cells contained multiple gene rearrangments; intact floxed-exons 5-12, intact floxed-neo cassette, or excision of both the exons and the neo cassette. Correctly targeted ES cells, containing only the floxed-exons, were injected into C57BL/6NCrl blastocysts and resulting chimeric males were bred with C57BL/6NCrl females. Resulting offspring were bred to C57BL/6NCrl for at least 2 generations. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Rene St-Arnaud|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||ILK(lox); ILKfl; ILKflox; ilkloxP|
|Gene Symbol and Name||Ilk, integrin linked kinase|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||LoxP sites were inserted downstream of exon 4 and exon 12 (along with a PGK-neomycin containing cassette).|
When maintaining a live colony mice homozygous for the floxed allele may be bred together.
When using the B6N;129-Ilktm1Star/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023310 in your Materials and Methods section.