This targeted mutation strain carries a knockout of Rgs16 (regulator of G-protein signaling 16). Mice on a mixed C57BL6 and SJL genetic background show a mild increase in fatty acid oxidation rates and plasma β-ketone levels after a 12-hour fast. This C57BL/6 background has not been assayed, as yet.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Thomas Wilkie, UT Southwestern
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Rgs16 | regulator of G-protein signaling 16 |
Regulators of G protein signaling (RGS) are GTPase-activating proteins for Gi and Gq α-subunits that control the intensity and duration of G protein-coupled receptor (GPCR) signaling. The associated pathways control glucose and fatty acid metabolism and the onset of obesity and diabetes.
This targeted mutation strain carries a knockout of Rgs16 (regulator of G-protein signaling 16) gene as confirmed by Western blot analysis. Mice on a mixed C57BL6 and SJL genetic background show a mild increase in fatty acid oxidation rates and plasma β-ketone levels after a 12-hour fast at the end of Zeitgeber time 12 (ZT12) light phase. These mice have been backcrossed to C57BL/6 for at least 10 generations, and the phenotype has not been confirmed.
LoxP sites were introduced to either side of exon 5 and a FRT-flanked PGK-neomycin cassette was placed in intron 5 by homologous recombination in 129X1/SvJ-derived MJ-1 embryonic stem (ES) cells (made by Bob Hammer, UT Southwestern). Exon 5 was excised through crosses with a C57BL/6 background Meox-cre strain. The FRT-flanked PGK-neo cassette is retained in the 3' UTR. Resultant mice were backcrossed to C57BL/6 for 10 generations by the donating laboratory.
Allele Name | targeted mutation 1, Thomas M Wilkie |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Rgs16, regulator of G-protein signaling 16 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 1 |
General Note | ES cell line = MJ-1 |
Molecular Note | A loxP site was inserted upstream of exon 5. An FRT-flanked neomycin resistance cassette with a loxP site was inserted downstream of exon 5. Cre-mediated recombination removed exon 5. The absence of protein expression was confirmed by western blot analysis. |
Homozyotes and heteroygotes are viable and fertile.
When using the B6.129X1(Cg)-Rgs16tm1Tmw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023204 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Rgs16<tm1Tmw> |
Frozen Mouse Embryo | B6.129X1(Cg)-Rgs16<tm1Tmw>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1(Cg)-Rgs16<tm1Tmw>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1(Cg)-Rgs16<tm1Tmw>/J | $3373.50 |
Frozen Mouse Embryo | B6.129X1(Cg)-Rgs16<tm1Tmw>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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