B6.129X1(Cg)-Rgs16^tm1Tmw/J

Stock number: 023204
Research areas: Diabetes and Obesity Research, Metabolism Research

Description

This targeted mutation strain carries a knockout of Rgs16 (regulator of G-protein signaling 16). Mice on a mixed C57BL6 and SJL genetic background show a mild increase in fatty acid oxidation rates and plasma β-ketone levels after a 12-hour fast. This C57BL/6 background has not been assayed, as yet.

Detailed description

Regulators of G protein signaling (RGS) are GTPase-activating proteins for G_i and G_q α-subunits that control the intensity and duration of G protein-coupled receptor (GPCR) signaling. The associated pathways control glucose and fatty acid metabolism and the onset of obesity and diabetes.

This targeted mutation strain carries a knockout of Rgs16 (regulator of G-protein signaling 16) gene as confirmed by Western blot analysis. Mice on a mixed C57BL6 and SJL genetic background show a mild increase in fatty acid oxidation rates and plasma β-ketone levels after a 12-hour fast at the end of Zeitgeber time 12 (ZT12) light phase. These mice have been backcrossed to C57BL/6 for at least 10 generations, and the phenotype has not been confirmed.

Development

LoxP sites were introduced to either side of exon 5 and a FRT-flanked PGK-neomycin cassette was placed in 3' of exon 5 by homologous recombination in 129X1/SvJ-derived MJ-1 embryonic stem (ES) cells (made by Bob Hammer, UT Southwestern). Exon 5 was excised through crosses with a C57BL/6 background Meox-cre strain. The FRT-flanked PGK-neo cassette is retained in the 3' UTR. Resultant mice were backcrossed to C57BL/6 for 10 generations by the donating laboratory.

Allele

Symbol: Rgs16^tm1Tmw
Name: targeted mutation 1, Thomas M Wilkie
MGI accession ID: MGI:5004805
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Rgs16
Marker name: regulator of G-protein signaling 16
Chromosome: 1
Strain of origin: 129X1/SvJ
Molecular note: A loxP site was inserted upstream of exon 5. An FRT-flanked neomycin resistance cassette with a loxP site was inserted downstream of exon 5. Cre-mediated recombination removed exon 5. The absence of protein expression was confirmed by western blot analysis.
General note: ES cell line = MJ-1