This targeted mutation strain carries a knockout of Rgs16 (regulator of G-protein signaling 16). Mice on a mixed C57BL6 and SJL genetic background show a mild increase in fatty acid oxidation rates and plasma β-ketone levels after a 12-hour fast. This C57BL/6 background has not been assayed, as yet.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Thomas Wilkie, UT Southwestern
Regulators of G protein signaling (RGS) are GTPase-activating proteins for Gi and Gq α-subunits that control the intensity and duration of G protein-coupled receptor (GPCR) signaling. The associated pathways control glucose and fatty acid metabolism and the onset of obesity and diabetes.
This targeted mutation strain carries a knockout of Rgs16 (regulator of G-protein signaling 16) gene as confirmed by Western blot analysis. Mice on a mixed C57BL6 and SJL genetic background show a mild increase in fatty acid oxidation rates and plasma β-ketone levels after a 12-hour fast at the end of Zeitgeber time 12 (ZT12) light phase. These mice have been backcrossed to C57BL/6 for at least 10 generations, and the phenotype has not been confirmed.
LoxP sites were introduced to either side of exon 5 and a FRT-flanked PGK-neomycin cassette was placed in intron 5 by homologous recombination in 129X1/SvJ-derived MJ-1 embryonic stem (ES) cells (made by Bob Hammer, UT Southwestern). Exon 5 was excised through crosses with a C57BL/6 background Meox-cre strain. The FRT-flanked PGK-neo cassette is retained in the 3' UTR. Resultant mice were backcrossed to C57BL/6 for 10 generations by the donating laboratory.
|Allele Name||targeted mutation 1, Thomas M Wilkie|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Rgs16, regulator of G-protein signaling 16|
|Strain of Origin||129X1/SvJ|
|General Note||ES cell line = MJ-1|
|Molecular Note||A loxP site was inserted upstream of exon 5. An FRT-flanked neomycin resistance cassette with a loxP site was inserted downstream of exon 5. Cre-mediated recombination removed exon 5. The absence of protein expression was confirmed by western blot analysis.|
Homozyotes and heteroygotes are viable and fertile.
When using the B6.129X1(Cg)-Rgs16tm1Tmw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023204 in your Materials and Methods section.