This Brsk1 knockout strain is useful in studies of axonal differentiation.
Joshua R Sanes, Harvard University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Brsk1 | BR serine/threonine kinase 1 |
The protein encoded by Brsk1 is a serine/threonine-protein kinase that is involved in neuronal polarization, axon branching, and presynaptic differentiation in diverse neuronal populations. These mice carry a targeted mutation of the Brsk1 gene in which exon 1 is replaced by a floxed NEO cassette. Mice that are homozygous for the targeted mutation are viable, with reduced fertility but no overt phenotype. No gene product (mRNA) is detected by Northern blot analysis of brain tissue from homozygous animals.
When crossed to Brsk2 knock out mice, or to Brsk2 floxed mice (Stock No. 023199) and Cre recombinase expressing mice, the resulting double null homozygotes die shortly after birth due to respiratory failure.
A targeting vector containing a floxed NEO cassette was used to disrupt exon 1. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then crossed to C57BL/6 and CD-1 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, Joshua R Sanes |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | SADB- |
Gene Symbol and Name | Brsk1, BR serine/threonine kinase 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | A loxP-flanked neomycin resistance gene replaced the first coding exon. Northern blot of mutant brain samples demonstrated the lack of transcript. |
When maintaining a live colony, these mice can be bred as homozygotes, however, homozygotes exhibit reduced fertility.
When using the STOCK Brsk1tm1Jrs/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #023198 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Brsk1<tm1Jrs> |
Frozen Mouse Embryo | STOCK Brsk1<tm1Jrs>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Brsk1<tm1Jrs>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Brsk1<tm1Jrs>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Brsk1<tm1Jrs>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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