STOCK Brsk1^tm1Jrs/J

Stock number: 023198
Research areas: Developmental Biology Research, Neurobiology Research

Description

This Brsk1 knockout strain is useful in studies of axonal differentiation.

Detailed description

The protein encoded by Brsk1 is a serine/threonine-protein kinase that is involved in neuronal polarization, axon branching, and presynaptic differentiation in diverse neuronal populations. These mice carry a targeted mutation of the Brsk1 gene in which exon 1 is replaced by a floxed NEO cassette. Mice that are homozygous for the targeted mutation are viable, with reduced fertility but no overt phenotype. No gene product (mRNA) is detected by Northern blot analysis of brain tissue from homozygous animals.

When crossed to Brsk2 knock out mice, or to Brsk2 floxed mice (Stock No. 023199) and Cre recombinase expressing mice, the resulting double null homozygotes die shortly after birth due to respiratory failure.

Development

A targeting vector containing a floxed NEO cassette was used to disrupt exon 1. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were then crossed to C57BL/6 and CD-1 mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Brsk1^tm1Jrs
Name: targeted mutation 1, Joshua R Sanes
MGI accession ID: MGI:3575112
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Brsk1
Marker name: BR serine/threonine kinase 1
Chromosome: 7
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP-flanked neomycin resistance gene replaced the first coding exon. Northern blot of mutant brain samples demonstrated the lack of transcript.