Agonist-stimulated endocytosis of synaptic AMPA glutamate receptors is impaired in mice homozygous for the Caly knockout allele. These mice may be useful in studies of clathrin-mediated endocytosis in the central nervous system, neuronal synaptic vesicle trafficking and recycling, as well as neuregulin processing and AP-3 dependent axonal transport.
Clare Bergson, Georgia Health Sciences University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Caly | calcyon neuron-specific vesicular protein |
Calcyon neuron-specific vesicular protein regulates clathrin-mediated endocytosis and AP-3 dependent endosomal trafficking. These knock-out mice carry a targeted mutation of the Caly gene in which exons 3 through 6 have been excised. Agonist-stimulated endocytosis of synaptic AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) glutamate receptors (AMPARs), proteolytic processing of neuregulin 1, and axonal targeting of AP-3 cargos are impaired in the brains of homozygotes. Cultured hippocampal neurons isolated from homozygotes and CA1 region brain slices lack long-term synaptic depression (LTD). Mice that are homozygous for the targeted mutation are viable and fertile. No gene product (mRNA) is detected by RT-PCR analysis of brain tissue from homozygotes.
During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.
A targeting vector was used to insert an FRT site-flanked NEO cassette into intron 6 and loxP sites upstream of exon 3 and downstream of exon 6. The construct was electroporated into unspecified C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells, containing the Calfl allele, were injected into BALB/c blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice. Heterozygous Calcyonfl mice were crossed to generate homozygotes. Homozygotes were crossed to CMVCRE mice to excise exons 3 through 6 and the FRT-flanked NEO cassette. The mice were then backcrossed to C57BL/6 for at least 10 generations. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1.1, Clare Bergson |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cal- |
Gene Symbol and Name | Caly, calcyon neuron-specific vesicular protein |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 7 |
Molecular Note | Mice homozygous for the floxed Calytm1Cber allele were crossed with Tg(CMV-cre)1Cgn transgenic mice to generate null homozygotes that were then used for further breeding. No mRNA was detectable by RT-PCR on cortex and hind-brain extracts from homozygotes. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the CalyKO mouse strain in a publication, please cite the originating article(s) and include JAX stock #023129 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Caly<tm1.1Cber> |
Frozen Mouse Embryo | B6(Cg)-Caly<tm1.1Cber>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Caly<tm1.1Cber>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Caly<tm1.1Cber>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Caly<tm1.1Cber>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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