NOD.129P2(Cg)-Tcrb^tm1Mom/MnkaJ

Stock number: 023082
Product name: TCRbeta-
Strain synonyms: beta-, CBeta-, TCRb-, TCR-Cbeta-
Research areas: Diabetes and Obesity Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

Mice homozygous for the Tcrb^tm1Mom targeted mutation are deficient in alpha beta T-cell receptor and may be useful when studying diabetes.

Detailed description

Mice homozygous for the Tcrb^tm1Mom targeted mutation are viable and fertile. Mice are deficient in T cells expressing alpha beta T-cell receptor. The total number of cells in the thymus is ~8% that of wildtype; CD4⁺CD8⁺ cells ~6% of wildtype. The proportion of CD4^-CD8^- (IL2 receptor positive) cells increases to about 50% of the total cell number. Alpha beta thymocyte differentiation is blocked at an earlier stage than the Tcra^tm1Mom strain. There is normal differentiation of gamma delta thymocytes. Homozygous mice do not develop diabetes and pancreatic islets are free of lymphacytic infiltration at 30 weeks of age. Heterozygous mice develop diabetes and insulitis.

Development

The Tcrb^tm1Mom mutant was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A targeting replacement vector was used which resulted in a 15-kb deletion encompassing the Tcrb locus from J-beta-1.3 to downstream of C-beta-2. The mutant contains the Pgk-neo gene in reverse transcriptional orientation. The 129-derived E14 ES cell line was used. The C57BL/6J strain (Stock No. 002118) was generated by backcrossing mice carrying the Tcrb^tm1Mom mutation to C57BL/6J inbred mice (Stock No. 000664) for 12 generations. These mice were bred to mice carrying the Tg(DO11.10)10Dlo transgene, and subsequently to NOD/ShiLtJ mice (Stock No. 001976) for 12 generations to produce this Stock No. 023082. The Tg(DO11.10)10Dlo transgene was bred out of this strain.

Allele

Symbol: Tcrb^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:1857256
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: beta-; CBeta^-; TCRb^-; TCRbeta-; TCR-Cbeta-
Marker symbol: Tcrb
Marker name: T cell receptor beta chain
Marker synonyms: TCRbeta; Tib
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Molecular note: 15kb of sequence was replaced with a PGK-neo cassette. The deletion included one of the two D gene segments, ten of the twelve J gene segments, and both constant gene segments.