Ncr1gfp mice are useful when tracking natural killer cells during infection.
Ofer Mandelboim, The Hebrew University of Jerusalem
Genetic Background | Generation |
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N10+pN2F13
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Ncr1 | natural cytotoxicity triggering receptor 1 |
An internal ribosome entry site (IRES)-GFP (green fluorescent protein) fusion protein, replaces exons 5-7 of the natural cytotoxicity triggering receptor 1 (Ncr1 or NKp46), abolishing gene function. NCR1 is expressed specifically on natural killer (NK) cells and possibly on related innate lymphocytes. Homozygous mice are viable and fertile. These mice are more susceptible to influenza viral infection at the LD50 dose. The GFP reported reveals increased numbers of NK cells at sites of infection and decreased circulating NK cells during infection. This reporter also enables the identification of Interferon Producing Killer Dendritic Cells-like (IKDCs) as activated NK cells.
A targeting vector was designed to replace exons 5-7 of the natural cytotoxicity triggering receptor 1 (Ncr1) with an internal ribosome entry site (IRES)-GFP (green fluorescent protein) fusion protein followed by a loxP-flanked neomycin (neo) resistance cassette. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Chimeras were made by ES cell aggregation and chimeric mice were bred with C57BL/6-Tg(Zp3-cre)93Knw/J mice (Stock No. 003651) to delete the neo cassette. These Ncr1gfp mice were bred to C57BL/6 mice for at least 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory Repository, mice were bred with C57BL/6J mice (Stock No. 000664) for at least one generation.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Three of the markers throughout the genome were still segregating for 129 suggesting an incomplete backcross.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Ncr1 is expressed specifically on natural killer (NK) cells and possibly on related innate lymphocytes. |
Allele Name | targeted mutation 1, Ofer Mandelboim |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Ncr1gfp |
Gene Symbol and Name | Ncr1, natural cytotoxicity triggering receptor 1 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Ncr1 is expressed specifically on natural killer (NK) cells and possibly on related innate lymphocytes. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | A targeting vector was designed to replace exons 5-7 with an IRES-GFP-PGK-neo. The neo was removed via cre-mediated recombination. Absence of endogenous transcript was confirmed by RT-PCR. Fluorescence is more intense after excision by Tg(Prm-cre)58Og (129/Sv background) or by Tg(Sp3-cre)93Knw (C57BL/6 background). When allele is made congenic on 129 or C57BL/6 background, immune system phenotype is not altered by removal of PGK-neo or by background. |
When maintaining a live colony, homozygous mice may be bred together.
When using the Ncr1gfp mouse strain in a publication, please cite the originating article(s) and include JAX stock #022739 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ncr1<tm1Oman> |
Frozen Mouse Embryo | B6;129-Ncr1<tm1Oman>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Ncr1<tm1Oman>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Ncr1<tm1Oman>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Ncr1<tm1Oman>/J | $3373.50 |
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