B6.129S6-Plin1^tm1Asg/J

Stock number: 021887
Research areas: Internal/Organ Research, Metabolism Research, Research Tools

Description

Homozygotes for this targeted mutation of the Plin1, perilipin 1, gene exhibit decreased adipose mass and adipocyte size, and increased basal lipolysis. This mutant mouse strain may be useful in studies of lipolysis and lipid metabolism.

Detailed description

The Plin1, perilipin 1, gene encodes a protein that protectively coats lipid storage droplets in adipocytes. Mice that are homozygous for this targeted mutation, which disrupts exon 3 with a NEO cassette, are viable and fertile. No gene product (mRNA or protein) is detected by Northern or Western blot analysis. Homozygotes exhibit decreased adipocyte size and reduced adipose mass. Interscapular brown adipose tissue (IBAT) and white adipose tissue depot amount is reduced in homozygotes. In addition, IBAT depots are darker in color, have smaller lipid droplets and 47% less triacylglycerol amounts when compared to wildtype controls. Brown adipocytes isolated from homozygous mice have a higher basal glycerol and fatty acid release rate, which is not enhanced by norepinephrine.

Development

A targeting vector designed by Dr. Andrew S. Greenberg (Tufts University) containing a NEO cassette was used to disrupt exon 3. The construct was electroporated into unspecified 129/SvEvTac derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Chimeric animals were subsequently generated. The donating investigator reported that these mice were then backcrossed to C57BL/6J for 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Plin1^tm1Asg
Name: targeted mutation 1, Andrew S Greenberg
MGI accession ID: MGI:3710993
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Plin1
Marker name: perilipin 1
Chromosome: 7
Strain of origin: 129S6/SvEvTac
Molecular note: Exon 3 (nucleotides 3515-3887) was replaced with a neomycin resistance cassette, thereby disrupting the coding of all known mRNAs.