These Vil1-cre mice express Cre recombinase in villus and crypt epithelial cells of the small and large intestines and may be useful in studies of intestinal organogenesis. In addition, Vil1-cre transgenic mice from founder line 1000 are reported to be absent of Cre recombinase activity in gonads.
Deborah L Gumucio, University of Michigan
Genetic Background | Generation |
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N10+pN4F10
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Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$286.78 Domestic price for female 4-week |
315.29 Domestic price for breeder pair |
Villin-cre transgenic mice have the mouse villin 1 promoter directing expression of Cre recombinase to villus and crypt epithelial cells of the small and large intestines, in a pattern that closely resembles endogenous Vil1 expression. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. The donating investigator indicates that expression is generally continuous, but that a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil1 gene expression onset of 9.0 dpc.
Villin-cre transgenic mice from founder line 1000 (Stock No. 021504) are reported to be absent of Cre recombinase activity in gonads. This is in contrast to Villin-cre transgenic mice from founder line 997 (Stock No. 004586) that are reported to have a very low level (<1%) of cells with Cre recombinase activity in the testes.
Villin-cre transgenic mice from founder line 1000 (also called Vil1-Cre 1000, Vil-Cre 1000, Villin-Cre 1000, 12.4KbVilCre 1000 or VCre1000) are viable and fertile as hemizygotes. Homozygous Vil1-Cre 1000 mice are viable but are not healthy and die prematurely.
The 12.4KbVilCre transgene was designed by Dr. Blair Madison (University of Michigan) to have the 12.4 kb fragment of the mouse villin 1 gene, the Cre recombinase coding domain and a metallothionein polyadenylation site sequence. This transgene was injected into the pronuclei of fertilized C57BL/6J x SJL/J mouse eggs. Several founder animals were bred to C57BL/6 mice for germline transmission, and founder line 1000 (also called Vil1-Cre 1000, Vil-Cre 1000, Villin-Cre 1000, 12.4KbVilCre 1000 or VCre1000) was established. The donating investigator reports that Vil1-Cre 1000 mice were subsequently backcrossed to C57BL/6 mice for at least ten generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre-mediated recombination occurs in villus and crypt epithelial cells of the small and large intestines, closely patterning the endogenous gene expression. Expression is generally continuous, but a small amount of mosaicism is noted in the colon. |
Allele Name | transgene insertion 1000, Deborah L Gumucio |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | VCre1000; Vil-Cre 1000; Villin-Cre 1000 |
Gene Symbol and Name | Tg(Vil1-cre)1000Gum, transgene insertion 1000, Deborah L Gumucio |
Gene Synonym(s) | |
Promoter | Vil1, villin 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre-mediated recombination occurs in villus and crypt epithelial cells of the small and large intestines, closely patterning the endogenous gene expression. Expression is generally continuous, but a small amount of mosaicism is noted in the colon. |
Strain of Origin | C57BL/6J x SJL/J |
Chromosome | UN |
Molecular Note | A transgenic construct containing the coding domain of Cre recombinase, driven by a 12.4 kb fragment of the mouse villin 1 gene, and a metallothionein polyadenylation site sequence was injected into the pronuclei of fertilized C57BL/6J x SJL/J mouse eggs. Founder line 1000 was subsequently established. |
When maintaining a live colony, hemizygous mice may be bred together, to wildtype (noncarrier) mice from the colony, or to C57BL/6J inbred mice (Stock No. 000664). The donating investigator reports that homozygotes are viable but are not healthy and die prematurely.
When using the Vil-Cre 1000 mouse strain in a publication, please cite the originating article(s) and include JAX stock #021504 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Vil1-cre)1000Gum |
Frozen Mouse Embryo | B6.Cg-Tg(Vil1-cre)1000Gum/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Vil1-cre)1000Gum/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Vil1-cre)1000Gum/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Vil1-cre)1000Gum/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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