B6.129S4(Cg)-Dck^tm1.2Radu/J

Stock number: 021125
Product name: dck^flox
Research areas: Cell Biology Research, Research Tools

Description

These Dck^flox/flox mutant mice possess loxP sites flanking exon 3 of the deoxycytidine kinase (Dck) gene and useful for studying deoxyribonucleoside salvage and recycling during DNA replication.

Detailed description

These Dck^flox/flox mutant mice possess loxP sites flanking exon 3, encoding the catalytic domain, of the deoxycytidine kinase (Dck) gene. Deoxycytidine kinase is a gene involved in deoxyribonucleoside salvage metabolism for use in DNA replication. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 3 deleted in the cre-expressing tissues.

For example, when bred to B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054), resulting Dck deficient offspring exhibit severe defects in T cell, B cell, and erythroid development resulting from acute cell cycle arrest in early S-phase in rapidly proliferating progenitor populations.

Development

A targeting vector was designed to insert a loxP sites flanking exon 3 of the deoxycytidine kinase (Dck) gene, followed by a frt-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S4/SvJae-derived LW-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6NTac mice. Offspring were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. The donating investigator reported that these mice were bred to C57BL/6NTac mice for at least 4 more generations (see SNP note below). Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 27 markers throughout the genome suggested a 129 strain genetic background contribution, while 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Dck^tm1.2Radu
Name: targeted mutation 1.2, Caius G Radu
MGI accession ID: MGI:5449592
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dck
Marker name: deoxycytidine kinase
Chromosome: 5
Strain of origin: 129S4/SvJae
Molecular note: A targeting vector was designed to insert a loxP sites flanking exon 3, followed by a frt-flanked neomycin resistance (neo) cassette. Flp-mediated recombination remvoed the neo cassette and left exon 3 floxed.