These mice express Cre recombinase under the control of the phosphoglycerate kinase 1 promoter in all tissues of the progeny derived from the cre-transgenic mother.
Dr. Peter Lonai, The Weizmann Institute of Science
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These mice express Cre recombinase under the control of the phosphoglycerate kinase 1 (Pgk1) promoter. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in a widespread fashion in the tissues of progeny derived from mothers carrying the transgene. Cre activity commences at the diploid phase of genesis. Hemizygotes are viable and fertile. The donating investigator has not tried to make this strain homozygous.
A transgenic construct was designed by Dr. Peter Lonai (The Weizmann Institute of Science) to contain a cre Recombinase gene under the control of the phosphoglycerate kinase 1 (Pgk1) promoter. This construct was introduced into BALB/c x C57BL/6 F2 donor oocytes. SNP typing indicates that it is likely the transgene integrated into a Chr 4 locus of BALB/c origin. Resulting offspring were bred to C57BL/6J mice for at least 21 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression |
Allele Name | transgene insertion 1, Peter Lonai |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Cremat; PGK:Cre; PGKCre; PGK-Cre; PGK-Crem |
Gene Symbol and Name | Tg(Pgk1-cre)1Lni, transgene insertion 1, Peter Lonai |
Gene Synonym(s) | |
Promoter | Pgk1, phosphoglycerate kinase 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Strain of Origin | (BALB/c x C57BL/6)F2 |
Chromosome | 4 |
Molecular Note | This transgene directs the expression of Cre recombinase under the control of a phosphoglycerate kinase promoter, with integration site effects. Expression of this transgene is under dominant maternal control, probably due to cis acting effects at the integration site. SNP typing indicates that it is likely the transgene integrated into a Chr 4 locus of BALB/c origin. Activity is thought to commence at the diploid phase of oogenesis, and directs complete recombination in all tissues of progeny derived from mothers carrying the transgene. |
When maintaining a live colony, hemizygous mice may be bred together. The donating investigator has not tried to maintain a homozygous colony.
When using the B6.C-Tg(Pgk1-cre)1Lni/CrsJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #020811 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemiozygous or Non carrier for Tg(Pgk1-cre)1Lni |
Frozen Mouse Embryo | B6.C-Tg(Pgk1-cre)1Lni/CrsJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.C-Tg(Pgk1-cre)1Lni/CrsJ Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.C-Tg(Pgk1-cre)1Lni/CrsJ Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.C-Tg(Pgk1-cre)1Lni/CrsJ Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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