B6.C-Tg(Pgk1-cre)1Lni/CrsJ

Stock number: 020811

Description

These mice express Cre recombinase under the control of the phosphoglycerate kinase 1 promoter in all tissues of the progeny derived from the cre-transgenic mother.

Detailed description

These mice express Cre recombinase under the control of the phosphoglycerate kinase 1 (Pgk1) promoter. When crossed with a strain containing a loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in a widespread fashion in the tissues of progeny derived from mothers carrying the transgene. Cre activity commences at the diploid phase of genesis. Hemizygotes are viable and fertile. The donating investigator has not tried to make this strain homozygous.

Development

A transgenic construct was designed by Dr. Peter Lonai (The Weizmann Institute of Science) to contain a cre Recombinase gene under the control of the phosphoglycerate kinase 1 (Pgk1) promoter. This construct was introduced into BALB/c x C57BL/6 F2 donor oocytes. SNP typing indicates that it is likely the transgene integrated into a Chr 4 locus of BALB/c origin. Resulting offspring were bred to C57BL/6J mice for at least 21 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Pgk1-cre)1Lni
Name: transgene insertion 1, Peter Lonai
MGI accession ID: MGI:2178050
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Pgk1-cre)1Lni
Marker name: transgene insertion 1, Peter Lonai
Chromosome: 4
Strain of origin: (BALB/c x C57BL/6)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Molecular note: This transgene directs the expression of Cre recombinase under the control of a phosphoglycerate kinase promoter, with integration site effects. Expression of this transgene is under dominant maternal control, probably due to cis acting effects at the integration site. SNP typing indicates that it is likely the transgene integrated into a Chr 4 locus of BALB/c origin. Activity is thought to commence at the diploid phase of oogenesis, and directs complete recombination in all tissues of progeny derived from mothers carrying the transgene.