These double H2KbDb KO mice carry knockout alleles of the MHC class I genes and exhibit decreased susceptibility to viral infection, as well as enhanced motor learning. They are suitable for use in applications related to the study of adaptive immunity, cytoxic T cell response and cerebellar synaptic plasticity.
Dr. Derry Roopenian, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | H2-K1 | histocompatibility 2, K1, K region |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | H2-D1 | histocompatibility 2, D region locus 1 |
These H-2 KbDb knock-out mice carry knock-out alleles of the MHC class I genes (Kb null and Db null) and lack CD8+ TCRalpha/beta T-cells, but have more CD4-positive, alpha beta T cells, and fewer single positive CD8 peripheral T-cells. Mice homozygous for both alleles exhibit diminished innate immune response, have decreased susceptibility to Lymphocytic Choriomeningitis Virus infection, and have an increased cytoxic T cell response to Listeria monocytogenes infection.
These mutant mice exhibit enhanced motor learning, with lowered threshold for induction of long-term depression at parallel fiber- Purkinje cell synapses.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The H2-K1tm1Bpe knockout allele (Kb null) and H2-D1tm1Bpe knockout allele (Db null) were both created by Dr. Beatrice Perarnau (Institut Pasteur, Paris France).
The Kb null allele has a hypoxanthine phosphoribosyl transferase (HPRT) minigene in the opposite transcriptional orientation replacing the second and third exons (which encode the α1-α2 peptide-presenting domains) of the H2-Kb locus (H2-K1) on chromosome 17. The construct was electroporated into 129P2/OlaHsd-Hprtb-m3-derived E14TG2a embryonic stem (ES) cells. Mutant animals were outcrossed to C57BL/6J. Kb null animals on a mixed B6;129 genetic background were used as described below.
The Db null allele has a lacZ gene and a neomycin resistance gene replacing the first three exons of the H2-Db locus (H2-D1) on chromosome 17. The construct was electroporated into 129P2/OlaHsd-derived CGR-8 embryonic ES cells. Mutant animals were outcrossed to C57BL/6J. The Db null animals on a mixed B6;129 genetic background were bred to B6;129-Kb null mice to generate the B6;129-Db Kb double knockout line.
Dr. Derry Roopenian obtained double knock-out mice that had been backcrossed to C57BL/6 for 9 generations, and then further backcrossed the mice to N11 on C57BL/6J. The mice were then maintained as double homozygotes before being cryopreserved as sperm. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes (Stock No. 000664).
Allele Name | targeted mutation 1, Beatrice Perarnau |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | H2-Kb-; H-2Ko; K-; Kb- |
Gene Symbol and Name | H2-K1, histocompatibility 2, K1, K region |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 17 |
Molecular Note | The central part of the H2-K gene, including the second and third exons was replaced by a HPRT minigene. |
Allele Name | targeted mutation 1, Beatrice Perarnau |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | D-; Db-; H-2 Dbo; H-2Db-; H-2Db knockout; H-2Do; HHD |
Gene Symbol and Name | H2-D1, histocompatibility 2, D region locus 1 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 17 |
Molecular Note | Exons 1, 2 and 3 of the b haplotype gene were replaced with plasmid sequences and a neomycin cassette. |
When maintaining a live colony, these mice can be bred as double homozygotes.
When using the double H2KbDb knockout mouse strain in a publication, please cite the originating article(s) and include JAX stock #019995 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterzygous for H2-K1<tm1Bpe> and Heterozygous for H2-D1<tm1Bpe> |
Frozen Mouse Embryo | B6.129P2-H2-K1<tm1Bpe> H2-D1<tm1Bpe>/DcrJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-H2-K1<tm1Bpe> H2-D1<tm1Bpe>/DcrJ Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-H2-K1<tm1Bpe> H2-D1<tm1Bpe>/DcrJ Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2-H2-K1<tm1Bpe> H2-D1<tm1Bpe>/DcrJ Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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