These knockout/knockin mice express β-galactosidase (lacZ) from a disrupted Sphk2 gene and exhibit a mild anaphylaxis when challenged. They maybe useful for studying the role of sphingosine-1-phosphate in cell survival and proliferation.
Richard L Proia, National Institutes of Health
An internal ribosome entry site (IRES) followed by a β-galactosidase (lacZ)-neomycin resistance (neo) cassette replaces part of exon 4 and exons 5-7 of the sphingosine kinase 2 (Sphk2) gene, abolishing gene function. SPHK2, expressed in many cell types, is an enzyme that catalyzes the synthesis of sphingosine-1-phosphate (S1P), a lipid signaling molecule, from sphingosine and ATP. S1P regulates immune cell trafficking and vascular function. Mice homozygous for this allele are viable and fertile. SPHK2 KO mice exhibit mild anaphylaxis when challenged, but recover quickly. When bred to SPHK1 KO mice (Stock No. 019095) resulting double knockout mice die by E13.5 due to developmental defects and hemorrhaging.
A targeting vector was designed to replace part of exon 4 and exons 5-7 of the sphingosine kinase 2 (Sphk2) gene with an internal ribosome entry site (IRES) followed by a β-galactosidase (lacZ)-neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6NCrl females. These mice were backcrossed to C57BL/6NCrl mice for at least 7 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Site of Expression|
|Allele Name||targeted mutation 1, Richard L Proia|
|Allele Type||Targeted (Reporter, Null/Knockout)|
|Allele Synonym(s)||targeted mutation 1, Richard L Proia; Sphk2tm1Rlp|
|Gene Symbol and Name||Sphk2, sphingosine kinase 2|
|Gene Synonym(s)||SK 2; SK-2; expressed sequence C76851; C76851; SPK 2; SPK-2|
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A lacZ-neo cassette replaced half of exon 4 through the end of exon 7 by homologous recombination. RT-PCR analysis of total RNA form adult tissues failed to detect transcript in mutants.|
|Mutations Made By|| |
Richard Proia, National Institutes of Health
When maintaining a live colony, homozygotes may be bred together.
When using the SphK2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #019140 in your Materials and Methods section.
|Hemizygous or Non carrier for Sphk2<tm1Rlp>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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