NOD.129P2(B6)-Lyz2^tm1(cre)Ifo/NadlJ

Stock number: 019096
Product name: LysMcre
Research areas: Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

The LysMcre knock-in/knockout allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene (Lyz2); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements. These LysMcre mice may be useful for Cre-lox studies of the myeloid cell lineage (monocytes, mature macrophages and granulocytes) and the innate immune response.

Detailed description

This strain expresses Cre recombinase from the endogenous Lyzs locus in myeloid cells. When crossed with a strain containing loxP site-flanked sequence, Cre-mediated recombination results in deletion of the flanked region in the myeloid cell lineage, including monocytes, mature macrophages, and granulocytes. Mice that are homozygous for the targeted mutation are viable, and fertile. This strain represents an effective tool for generating myeloid cell-specific targeted mutants.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector containing a FRT-flanked neomycin resistance gene, herpes simplex virus thymidine kinase genes and NLS (nuclear localization signal) -Cre cDNA, was inserted into the endogenous ATG-start site within exon 1 of the Lyzs gene. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Flp expression vector for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Flp recombination were injected in blastocysts. These mice were maintained on a C57BL/6 background as Stock No. 004781. Dr. Jerry Nadler from Eastern Virginia Medical School subsequently backcrossed this strain to NOD/ShiLtJ mice (Stock No. 001976) for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to NOD/ShiLtJ mice for at least one generation to establish the colony.

Allele

Symbol: Lyz2^tm1(cre)Ifo
Name: targeted mutation 1, Irmgard Forster
MGI accession ID: MGI:1934631
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Lyz2
Marker name: lysozyme 2
Chromosome: 10
Strain of origin: 129P2/OlaHsd
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: myeloid cells - including monocytes, mature macrophages, and granulocytes
Molecular note: An NLS-cre gene was inserted into the endogenous ATG start site of the gene. An frt-flanked neomycin cassette was removed in ES cells via Flp-mediated recombination prior to production of chimeric mice.