These mice contain loxP flanked transcription blocker (loxTB) sequence between exons 16 and 17 of the leptin receptor (Lepr) gene, abolishing transcription of the exons downstream of the loxTB. Leptin is an adipose-derived hormone that acts to regulate food intake, energy expenditure, and body weight. LeprloxTB mice, lacking the long (B) isoform of the leptin receptor, are obese, hyperglycemic, and hyperinsulinemic, and have reduced energy expenditure. Mice that are heterozygous for the targeted mutation are viable and fertile, while homozygous mice are infertile. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have the loxTB removed and Lepr expression will be restored in cre expressing cells. 

For example, when bred to STOCK Tg(Pomc1-cre)16Lowl/J mice (Stock No. <a href="https://www.jax.org/strain/005965">005965</a>) expressing Cre-recombinase in proopiomelanocortin (POMC) neurons, the resulting offspring have normal glucose and glucagon levels. They have moderately restored energy expenditure and body weight, with no reduction on food intake.

LeprloxTB mice only express the leptin receptor after Cre-mediated recombination.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

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