The CD19-Cre knockin/knockout allele has a Cre recombinase gene inserted into the first coding exon of the CD19 antigen gene (Cd19); both abolishing endogenous Cd19 gene function and placing cre expression under the control of the endogenous Cd19 promoter/enhancer elements. Cre recombinase expression is directed at the earliest stages and throughout B-lymphocyte development and differentiation. Homozygous mice are also useful for studying B cell-deficiency.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Cd19 | CD19 antigen |
To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.
The parental line, C57BL/6-congenic CD19-Cre knockin/knockout mice, are available and described as Stock No. 006785. It should be noted that the phenotype of these C57BL/6NJ-congenic CD19-Cre knockin/knockout mice (Stock No. 018958) could vary from that of the C57BL/6-congenic parental line from which it was derived. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.
Mice harboring the CD19-Cre knockin/knockout allele on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 2007 (as Stock No. 006785). In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018958). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | B cells |
Allele Name | targeted mutation 1, University of Cologne |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | B-Cre; CD19-; Cd19Cre; Cd19cre; CD19-Cre; CD19-KO |
Gene Symbol and Name | Cd19, CD19 antigen |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | B cells |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | In frame insertion of a cre recombinase gene into the first coding exon followed by an frt-flanked neomycin cassette. A frameshift mutation was also introduced into exon 5. This allele expresses cre recombinase specifically in B lineage cells throughout development. |
Mutations Made By | Robert Rickert, The Burnham Institute |
Mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain. In 2007, the donating investigator of the C57BL/6-congenic CD19-Cre strain (Stock No. 006785) reported that homozygotes did not breed well after three months of age. The C57BL/6-congenic homozygous breeding pairs maintained at The Jackson Laboratory Repository have shown no such problems. Therefore, when maintaining the live C57BL/6N-congenic colony (Stock No. 018958), homozygous mice may be bred together.
When using the Cd19cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #018958 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Cd19<tm1(cre)Cgn> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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