B6N.129P2-Cd19^tm1(cre)Cgn/J

Stock number: 018958
Product name: Cd19cre
Research areas: Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

The CD19-Cre knockin/knockout allele has a Cre recombinase gene inserted into the first coding exon of the CD19 antigen gene (Cd19); both abolishing endogenous Cd19 gene function and placing cre expression under the control of the endogenous Cd19 promoter/enhancer elements. Cre recombinase expression is directed at the earliest stages and throughout B-lymphocyte development and differentiation. Homozygous mice are also useful for studying B cell-deficiency.

Detailed description

To be more suitable for use with C57BL/6N-congenic Knockout Mouse Project (KOMP) strains with floxed alleles, The Jackson Laboratory Repository chose several Cre recombinase-expressing strains and backcrossed them onto the C57BL/6N genetic background using a marker-assisted, speed-congenic approach. This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.

The parental line, C57BL/6-congenic CD19-Cre knockin/knockout mice, are available and described as Stock No. 006785. It should be noted that the phenotype of these C57BL/6NJ-congenic CD19-Cre knockin/knockout mice (Stock No. 018958) could vary from that of the C57BL/6-congenic parental line from which it was derived. We may modify the C57BL/6NJ-congenic strain description if necessary as published results become available.

Development

Mice harboring the CD19-Cre knockin/knockout allele on a C57BL/6 genetic background were sent to The Jackson Laboratory Repository in 2007 (as Stock No. 006785). In 2012, some mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations using a marker-assisted, speed congenic approach to generate this C57BL/6NJ-congenic strain (Stock No. 018958). This approach employed 148 single nucleotide polymorphism (SNP) markers that differ between the C57BL/6N and C57BL/6J substrains, covering all 19 chromosomes and the X chromosome. This analysis determined that the colony has at least 99% of the SNP markers as C57BL/6N allele-type.

Allele

Symbol: Cd19^tm1(cre)Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:1931143
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Cd19
Marker name: CD19 antigen
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: B cells
Molecular note: In frame insertion of a cre recombinase gene into the first coding exon followed by an frt-flanked neomycin cassette. A frameshift mutation was also introduced into exon 5. This allele expresses cre recombinase specifically in B lineage cells throughout development.