These floxed Itga2 (α2flox) mice possess loxP sites flanking exon 1 of the integrin alpha 2 gene (Itga2), and may be useful in Cre-lox studies of platelet function and thrombosis.
Thomas J. Kunicki, CHOC Children's Hospital
Integrin alpha 2 is a receptor for collagens, laminin, decorin and collagen related-peptides, and is involved in platelet and cell adhesion to the extracellular matrix. These mice possess loxP sites on either side of exon 1 of the targeted gene. Mice that are homozygous for this allele are viable & fertile. When these mutant mice are bred to others that express Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues.
When bred to a strain with Cre recombinase expression in megakaryocytes (see Stock No. 008535 for example), this mutant mouse strain may be useful in studies of platelet function.
A loxP site-flanked targeting vector containing a frt-flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted upstream of exon 1. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into (BALB/cByJ x B6(Cg)-Tyrc-2J/J)F2 blastocysts. The resulting chimeric animals were crossed to transgenic mice (on the C57BL/6 genetic background) expressing FLP1 recombinase under the control of the beta-actin promoter (Stock No. 003800) that had been backcrossed for 13 to 14 generations onto C57BL/6 albino mice (B6(Cg)-Tyrc-2J/J; Stock No. 000058) to remove the frt-flanked selection cassette. The resulting mice were backcrossed to C57BL/6 for more than six generations (see SNP results below) prior to sending homozygous mice to The Jackson Laboratory Repository in 2012. Upon arrival, the mice were crossed to C57BL/6J inbred mice (Stock No. 000664) at least once to establish our colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. This revealed 2 markers on chromosome 11 and 1 marker on chromosome 19 that were segregating for allele-type markers other than C57BL/6. These data suggest the mice have a BALB/c contribution.
|Allele Name||targeted mutation 1.1, Thomas Kunicki|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||floxed Itga2; floxed-alpha2|
|Gene Symbol and Name||Itga2, integrin alpha 2|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||A loxP site-flanked targeting vector containing a FRT-flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 1 of the targeted gene, and another loxP site was inserted upstream of exon 1. Flp-mediated recombination removed the neo cassette and left exon 2 floxed.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.Cg-Itga2tm1.1Tkun/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018921 in your Materials and Methods section.