This strain may be useful for generating conditional mutants for applications related to embryonic development and thymopoiesis. These mice possess loxP sites flanking exon 17 of the Trpm7 gene, a transient receptor potential (TRP) cation-permeant ion channel.
David Clapham, Boston Children's Hospital, Harvard University
TRPM7 is a transient receptor potential (TRP) cation-permeant ion channel that functions as an ion channel and a kinase. TRPM7 is expressed in the early embryo, predominantly in the fetal heart at E9.5, but expanding throughout the embryo by E14.5. These mutant mice possess loxP sites flanking exon 17 of the (Trpm7) gene. Mice that are homozygous for this allele are viable and fertile. When bred to mice that express Cre recombinase, the resulting offspring will have exon 17 deleted in the cre-expressing tissues. These mice may be useful in generating conditional knockouts for studying the role of embryogenesis and thymopoiesis.
For example, when bred to mice carrying Tg(Lck-cre)548 Jxm (see Stock No. 003802), Cre recombinase expression in thymocytes results in a block in thymopoiesis at the double-negative stage, depletion of thymic medullary cells, reduced numbers of T cells and aberrant synthesis of several growth factors.
When bred to mice carrying Tg(Sox2-cre)1Amc (see Stock No. 008454), Cre recombinase expression in the developing embryo results in embryonic lethality.
A targeting vector was designed to insert a loxP site upstream of exon 17 followed by a second loxP site and a frt-flanked neomycin resistance (neo) cassette downstream of exon 17. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric mice were bred to 129S6/SvEvTac mice. Offspring were bred with FLPe transgenic mice of unknown genetic background to delete the neo cassette. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, David E Clapham|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Trpm7, transient receptor potential cation channel, subfamily M, member 7|
|Gene Synonym(s)||2310022G15Rik; 2310022G15Rik; 4833414K03Rik; 4833414K03Rik; 5033407O22Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Chak; LTRPC7; LTrpC-7; Ltpr7; Ltrpc7; RIKEN cDNA 2310022G15 gene; RIKEN cDNA 4833414K03 gene; RIKEN cDNA 5033407O22 gene; TRP-PLIK; Trp-plik|
|Strain of Origin||129S4/SvJae|
|Molecular Note||LoxP sites were inserted around exon 17 and an frt-flanked neo cassette was inserted within the floxed locus. The neo cassette was removed by in vivo flp mediated recombination.|
|Mutations Made By|| |
David Clapham, Boston Children's Hospital, Harvard University
When maintaining a live colony, these mice are bred as homozygotes.
When using the STOCK Trpm7tm1Clph/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018784 in your Materials and Methods section.
|Heterozygous for Trpm7<tm1Clph>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
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