These PWS-ICflox6kb mice may be useful in generating conditional mutations for studying the role of Snrpn and the temporal and developmental requirements of the Prader-Willi imprinting center.
James Resnick, University of Florida
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Snrpn | small nuclear ribonucleoprotein N |
Mice homozygous for this PWS-ICflox6kb allele are viable and fertile, with loxP sites flanking the 6 kb Prader-Willi Syndrome imprinting center (PWS-IC) region around exon 1 of the Snrpn gene . When bred to mice that express Cre recombinase, the resulting offspring will have this area deleted in the cre-expressing tissue(s). Because Snrpn is imprinted and only expressed from the paternal allele, breeding PWS-ICflox6kb males with cre-expressing females is required to generate deleted offspring with the knockout phenotype. These PWS-ICflox6kb mice be useful in studying the temporal and spatial functions of the Prader-Willi imprinting center.
When crossed to female mice carrying the Tg(CMV-cre)1Cgn allele (see Stock No. 006054), widespread Cre recombinase expression prior to implantation results in postnatal lethality.
When crossed to female mice carrying the Tg(Nes-cre)1Kln allele (see Stock No.003771), Cre recombinase expression in brain precursors cells starting at E10.5 results in a growth retardation, but is not lethal.
A targeting vector was designed to insert a loxP site upstream of exon 1 followed by a loxP-flanked neomycin resistance (neo) cassette downstream of exon 1. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing plasmid to remove the neo selection cassette, but retain the loxP-flanked 6 kb Prader-Willi Syndrome-imprinting center (PWS-IC) region centered around exon 1. Chimeric mice crossed to C57BL/6J and offspring were backcrossed to C57BL/6J for 11 generations.
Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 2.1, Karen A Johnstone |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | PWS-ICflox6kb |
Gene Symbol and Name | Snrpn, small nuclear ribonucleoprotein N |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 7 |
Molecular Note | A loxP site was inserted 3.7 kb upstream of exon 1. A floxed neo cassette was inserted downstream of exon 1. Cre-mediated recombination first removed the neo cassette and left the 6 kb encompassing the Prader-Willi syndrome imprinting center (PWS-IC) floxed. |
Mutations Made By | James Resnick, University of Florida |
While maintaining a live colony, these mice are bred as homozygotes.
Because Snrpn is imprinted and only expressed from the paternal allele, breeding PWS-ICflox6kb males with cre-expressing females is required to generate offspring with the deleted flanked sequence and the knockout phenotype.
When using the B6.129S1-Snrpntm2.1Kaj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018395 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Snrpn<tm2.1Kaj> |
Frozen Mouse Embryo | B6.129S1-Snrpn<tm2.1Kaj>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Snrpn<tm2.1Kaj>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Snrpn<tm2.1Kaj>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Snrpn<tm2.1Kaj>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.