These Txnip (thioredoxin interacting protein) knockout mice may be useful in studies of metabolism, angiogenesis, and cancer.
Richard T Lee, Harvard Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Txnip | thioredoxin interacting protein |
The Txnip (thioredoxin interacting protein) gene produces a redox-sensitive signaling protein that participates in a variety of biological functions that are still being characterized.
This strain was created by breeding animals carrying a floxed exon 1 allele (see Stock No. 016847) with Sox2-cre mice to excise the targeted exon.
A similar knockout allele in which the same floxed exon was removed via crosses with a Prm-cre strain (Txniptm1.1Rlee) was published in Circulation Research, 2007 (PMID 17916779). Fasting blood glucose levels in their homozygous mice indicated hypoglycemia without hyperinsulinemia. When placed on a high-fat diet for 4 weeks, their homozygous mice showed significant increases in adipogenesis and insulin sensitivity as compared to wildtype controls. Their homozygotes were viable and fertile.
Although generated in a similar fashion, the offered Txniptm1.1Rlee allele has not been fully characterized.
A targeting construct incorporating a loxP-exon1-FRT-PGKneobpA-FRT-loxP sequence was introduced to J1 129S4/SvJae-derived embryonic stem (ES) cells. Resultant mice were crossed with 129Sv background animals expressing Gt(ROSA)26Sortm1(FLP1)Dym to excise the FRT-flanked neomycin cassette, leaving exon 1 flanked by loxP sites. This strain was maintained on a mixed 129 and C57BL/6 genetic background by the donating laboratory, and crossed with a cardiac-specific C57BL/6-129 Mer-Cre-Mer strain. This non-germline Cre has been bred away from the line to create Stock No. 016847. This strain was subsequently crossed with a second C57BL/6 background cre strain (Sox2-cre; see Stock No. 014094) to excise floxed exon 1 at The Jackson Laboratory. Animals were then backcrossed to C57BL6NJ (see Stock No. 005304) to breed cre out of the colony.
Allele Name | targeted mutation 1.1, Richard T Lee |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Txnipnull |
Gene Symbol and Name | Txnip, thioredoxin interacting protein |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 3 |
Molecular Note | Exon 1 was deleted via cre mediated recombination, Northern and western blot analysis confirmed the absence of mRNA and protein expression in the heart. |
Mutations Made By | Richard Lee, Harvard Medical School |
Heterozygotes and homozygotes are viable and fertile.
When using the B6;129-Txniptm1.1Rlee/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #018313 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Txnip<tm1.1Rlee> |
Frozen Mouse Embryo | B6;129-Txnip<tm1.1Rlee>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Txnip<tm1.1Rlee>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Txnip<tm1.1Rlee>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Txnip<tm1.1Rlee>/J Frozen Embryo | $3373.50 |
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