B6;129-Txnip^tm1.1Rlee/J

Stock number: 018313
Research areas: Research Tools

Description

These Txnip (thioredoxin interacting protein) knockout mice may be useful in studies of metabolism, angiogenesis, and cancer.

Detailed description

The Txnip (thioredoxin interacting protein) gene produces a redox-sensitive signaling protein that participates in a variety of biological functions that are still being characterized.

This strain was created by breeding animals carrying a floxed exon 1 allele (see Stock No. 016847) with Sox2-cre mice to excise the targeted exon.

A similar knockout allele in which the same floxed exon was removed via crosses with a Prm-cre strain (Txnip^tm1.1Rlee) was published in Circulation Research, 2007 (PMID 17916779). Fasting blood glucose levels in their homozygous mice indicated hypoglycemia without hyperinsulinemia. When placed on a high-fat diet for 4 weeks, their homozygous mice showed significant increases in adipogenesis and insulin sensitivity as compared to wildtype controls. Their homozygotes were viable and fertile.

Although generated in a similar fashion, the offered Txnip^tm1.1Rlee allele has not been fully characterized.

Development

A targeting construct incorporating a loxP-exon1-FRT-PGKneobpA-FRT-loxP sequence was introduced to J1 129S4/SvJae-derived embryonic stem (ES) cells. Resultant mice were crossed with 129Sv background animals expressing Gt(ROSA)26Sortm1(FLP1)Dym to excise the FRT-flanked neomycin cassette, leaving exon 1 flanked by loxP sites. This strain was maintained on a mixed 129 and C57BL/6 genetic background by the donating laboratory, and crossed with a cardiac-specific C57BL/6-129 Mer-Cre-Mer strain. This non-germline Cre has been bred away from the line to create Stock No. 016847. This strain was subsequently crossed with a second C57BL/6 background cre strain (Sox2-cre; see Stock No. 014094) to excise floxed exon 1 at The Jackson Laboratory. Animals were then backcrossed to C57BL6NJ (see Stock No. 005304) to breed cre out of the colony.

Allele

Symbol: Txnip^tm1.1Rlee
Name: targeted mutation 1.1, Richard T Lee
MGI accession ID: MGI:3818744
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Txnip
Marker name: thioredoxin interacting protein
Chromosome: 3
Strain of origin: 129S4/SvJae
Molecular note: Exon 1 was deleted via cre mediated recombination, Northern and western blot analysis confirmed the absence of mRNA and protein expression in the heart.