B6.129-Cul9^tm2Jdec/J

Stock number: 018059
Research areas: Research Tools

Description

These mice possess loxP sites flanking exons 4 through 6 of the Cul9, cullin 9, gene and may have applications in studies related to the ubiquitin-proteasome pathway pathway and protein neddylation.

Detailed description

Cullin 9, also known as PARC (PARkin-like cytoplasmic protein), is involved in the post-translational protein modification processes ubiquitination and neddylation through interactions with both NEDD8 and RBX1. These mice possess loxP sites on either side of exons 4 through 6 of the cullin 9 (Cul9) gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4 through 6 deleted in cre-expressing tissues. These mice carry a FRT site flanked NEO cassette. The Donating Investigator reports that this NEO cassette inhibits expression of the (Cul9) gene. When bred to FLP recombinase expressing mice, resulting offspring will exhibit a wildtype level of (Cul9)expression. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background.

When bred to a strain with early embryonic Cre recombinase expression (see Stock No. 003724 for example), this mutant mouse strain may be useful in studies of the ubiquitin-proteasome pathway and protein neddylation.

Development

A targeting vector containing a FRT site flanked NEO cassette and a loxP site was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 4 of the targeted gene, and another loxP site was inserted downstream of exon 6. This construct was electroporated into unspecified 129Sv derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred with C57BL/6 mice. The mice were subsequently backcrossed to C57BL/6 for 7 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Cul9^tm2Jdec
Name: targeted mutation 2, James A DeCaprio
MGI accession ID: MGI:5320484
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cul9
Marker name: cullin 9
Chromosome: 17
Strain of origin: 129
Molecular note: A targeting vector containing a FRT site flanked neo cassette and a loxP site was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 4 of the targeted gene, and another loxP site was inserted downstream of exon 6. This allele was used to generate Cul9^tm1Jdec.