ULK2 is an evolutionarily conserved serine threonine kinase which plays a role in the regulation of neurotransmitter trafficking and axonal growth. ULK2 is a homolog of yeast autophagy-related gene (Atg1), which has been implicated in organelle autophagy during cell growth and survival.These Ulk2FL mutant mice possess a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring will have exons 1-3, and the neo cassette, deleted in cre-expressing tissues. ULK2-deficient mice have no overt autophagy phenotype. When these floxed mice are bred to B6;129-Ulk1tm1Kund/J mice (Stock No. 017976), and subsequently to mice that express Cre recombinase, double KO mice die within 24 hours of birth. When mouse embryonic fibroblasts (MEFs) are cultured from these double KO mice, amino-acid deprivation does not induce autophagy, while normal autophagy is evident during glucose deprivation and ammonia induction.
A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3 of the Unc-51 like kinase 2 (Ulk2) gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred with C57BL/6J females. These mice were backcrossed to C57BL/6J mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Craig B Thompson|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ulk2, unc-51 like kinase 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 3.|
|Mutations Made By|| |
Craig Thompson, Memorial Sloan Kettering Cancer Center
When maintaining a live colony, homozygous mice may be bred together.
When using the Ulk2FL mouse strain in a publication, please cite the originating article(s) and include JAX stock #017977 in your Materials and Methods section.