LoxP sites flank exons 3-5 of the Calcineurin &beta;1 (Ppp3r1, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)) gene in these floxed mutant mice. When bred to Cre-recombinase mice, excision of the floxed exons can be directed in a tissue/cell-specific manner in the resulting offspring. Disruption of the calcineurin &beta;1 subunit results in a lack of calcineurin enzymatic activity in non-germline cell types. 
Ppp3r1 is a Ca2+-binding regulatory subunit of heterodimeric calcineurin, a Ca2+- and calmodulin-dependent serine/threonine protein phosphatase involved in a number of cellular processes and calcium-dependent signaling pathways.


When crossed with Lck-cre mice to specifically block calcineurin expression in thymocytes, it was shown that calcineurin &beta;1 is essential for positive but not negative selection during thymocyte development. 


 When bred to a strain expressing Cre recombinase in endothelial cells (see Stock No. <a href="https://www.jax.org/strain/008863">008863</a> for example), this mutant mouse strain may be useful in studies of angiogenesis.

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This strain carries a floxed allele of the Ppp3r1 gene. Cre excision disrupts calcineurin enzymatic activity in non-germline cell types. These mice have been useful in studies of T cell development/selection.

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