This strain carries a floxed allele of the Ppp3r1 gene. Cre excision disrupts calcineurin enzymatic activity in non-germline cell types. These mice have been useful in studies of T cell development/selection.
Gerald R Crabtree, Stanford University Medical Center
LoxP sites flank exons 3-5 of the Calcineurin β1 (Ppp3r1, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)) gene in these floxed mutant mice. When bred to Cre-recombinase mice, excision of the floxed exons can be directed in a tissue/cell-specific manner in the resulting offspring. Disruption of the calcineurin β1 subunit results in a lack of calcineurin enzymatic activity in non-germline cell types.
Ppp3r1 is a Ca2+-binding regulatory subunit of heterodimeric calcineurin, a Ca2+- and calmodulin-dependent serine/threonine protein phosphatase involved in a number of cellular processes and calcium-dependent signaling pathways.
When crossed with Lck-cre mice to specifically block calcineurin expression in thymocytes, it was shown that calcineurin β1 is essential for positive but not negative selection during thymocyte development.
When bred to a strain expressing Cre recombinase in endothelial cells (see Stock No. 008863 for example), this mutant mouse strain may be useful in studies of angiogenesis.
Exons 3-5 of the targeted gene were flanked by loxP sites and an FRT-flanked neomycin cassette was introduced to intron 3 using TC1 129S6/SvEvTac-derived embryonic stem (ES) cells. Transient transfection with a FLP-expressing vector excised the neomycin cassette. This strain was backcrossed to 129S1/SvImJ and maintained on a mixed C57BL/6-129S genetic background by the donating lab.
|Allele Name||targeted mutation 2, Gerald R Crabtree|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Cnb1f; Cnb1flox; CnB1-LoxP|
|Gene Symbol and Name||Ppp3r1, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exons 3 through 5 were flanked by single loxP sites. A single frt site remained in intron 3 following FLP-mediated excition of an frt-flanked neo cassette.|
|Mutations Made By|| |
Gerald Crabtree, Stanford University Medical Center
Homozygotes or heterozygotes may be bred.
When using the Cnb1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #017692 in your Materials and Methods section.