This strain carries a floxed allele of the Ppp3r1 gene. Cre excision disrupts calcineurin enzymatic activity in non-germline cell types. These mice have been useful in studies of T cell development/selection.
Gerald R Crabtree, Stanford University Medical Center
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ppp3r1 | protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) |
LoxP sites flank exons 3-5 of the Calcineurin β1 (Ppp3r1, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)) gene in these floxed mutant mice. When bred to Cre-recombinase mice, excision of the floxed exons can be directed in a tissue/cell-specific manner in the resulting offspring. Disruption of the calcineurin β1 subunit results in a lack of calcineurin enzymatic activity in non-germline cell types.
Ppp3r1 is a Ca2+-binding regulatory subunit of heterodimeric calcineurin, a Ca2+- and calmodulin-dependent serine/threonine protein phosphatase involved in a number of cellular processes and calcium-dependent signaling pathways.
When crossed with Lck-cre mice to specifically block calcineurin expression in thymocytes, it was shown that calcineurin β1 is essential for positive but not negative selection during thymocyte development.
When bred to a strain expressing Cre recombinase in endothelial cells (see Stock No. 008863 for example), this mutant mouse strain may be useful in studies of angiogenesis.
Exons 3-5 of the targeted gene were flanked by loxP sites and an FRT-flanked neomycin cassette was introduced to intron 3 using TC1 129S6/SvEvTac-derived embryonic stem (ES) cells. Transient transfection with a FLP-expressing vector excised the neomycin cassette. This strain was backcrossed to 129S1/SvImJ and maintained on a mixed C57BL/6-129S genetic background by the donating lab.
Allele Name | targeted mutation 2, Gerald R Crabtree |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Cnb1f; Cnb1flox; CnB1-LoxP |
Gene Symbol and Name | Ppp3r1, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 11 |
Molecular Note | Exons 3 through 5 were flanked by single loxP sites. A single frt site remained in intron 3 following FLP-mediated excition of an frt-flanked neo cassette. |
Mutations Made By | Gerald Crabtree, Stanford University Medical Center |
Homozygotes or heterozygotes may be bred.
When using the Cnb1flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #017692 in your Materials and Methods section.
Facility Barrier Level Descriptions
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Heterozygous for Ppp3r1<tm2Grc> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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