These double floxed mutant mice possess loxP sites flanking exons 4-5 of the Stk4 gene and exons 5-6 of the Stk3 gene. This strain may be useful for generating conditional mutations in applications related to studies of cell proliferation, apoptosis, and tumorigenesis.
Randy L Johnson, M.D. Anderson Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Stk4 | serine/threonine kinase 4 |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Stk3 | serine/threonine kinase 3 |
Stk4 (serine/threonine kinase 4 ) and Stk3 (serine/threonine kinase 3) are the mouse orthologs for the Drosophila hippo gene. Both Stk4 and Stk3 are key components of the Salvador-Warts-Hippo (SWH) pathway, which regulates tissue growth and organ size by restricting cell proliferation and promoting apoptosis. Mice that are homozygous for both the floxed targeted mutations are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 4-5 of the (Stk4) gene and exons 5-6 of the (Stk3) gene deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in the liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte proliferation and liver cancer.
For the Stk4tm1.1Rjo (mst1) targeted mutation, a targeting vector containing an FRT flanked NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 5 and loxP sites were inserted flanking exons 4 and 5. The construct was electroporated into (C57BL/6Brd-Tyrc-Brd x 129S6/SvEvTac)F1-derived RJ2.2 embryonic stem (ES) cells. ES cells containing the construct were aggregated with CD1 morulae and the resulting chimeric males were bred with CD1 females for germline transmission. The mice were then bred to a FLPe recombinase expressing strain to remove the selection cassette. Heterozygotes that retained the loxP site flanked exons 4 and 5 were then intercrossed to generation homozygotes.
For the Stk3tm1.1Rjo (mst2) targeted mutation, a targeting vector containing an FRT flanked NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 6 and loxP sites were inserted flanking exons 5 and 6. The construct was electroporated into (C57BL/6Brd-Tyrc-Brd x 129S6/SvEvTac)F1-derived RJ2.2 embryonic stem (ES) cells. ES cells containing the construct were aggregated with CD1 morulae and the resulting chimeric males were bred with CD1 females for germline transmission. The mice were then bred to a FLPe recombinase expressing strain to remove the selection cassette. Heterozygotes that retained the loxP site flanked exons 5 and 6 were then intercrossed to generation homozygotes.
The single mutant lines were then intercrossed to obtain this double mutant strain, which was maintained on a mixed background consisting of CD1, C57BL/6 and 129.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1.1, Randy L Johnson |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Stk4, serine/threonine kinase 4 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6Brd-Tyrc-Brd x 129S6/SvEvTac)F1 |
Chromosome | 2 |
Molecular Note | Exons 4 and 5 were floxed and an FRT flanked neo cassette placed in intron 5. The neo cassette was removed by flp-mediated recombination, leaving exons 4 and 5 flanked by loxP sites. |
Allele Name | targeted mutation 1.1, Randy L Johnson |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Stk3, serine/threonine kinase 3 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6Brd-Tyrc-Brd x 129S6/SvEvTac)F1 |
Chromosome | 15 |
Molecular Note | Exons 5 and 6 were floxed and an FRT flanked neo cassette was placed in intron 6. The neo cassette was removed by flp-mediated recombination, leaving exons 5 and 6 flanked by loxP sites. |
When maintaining a live colony, these mice can be bred as homozygotes for both alleles.
When using the mst1fl mst2fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #017635 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Stk4<tm1.1Rjo>,Heterozygous for Stk3<tm1.1Rjo> |
Frozen Mouse Embryo | STOCK Stk4<tm1.1Rjo> Stk3<tm1.1Rjo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Stk4<tm1.1Rjo> Stk3<tm1.1Rjo>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Stk4<tm1.1Rjo> Stk3<tm1.1Rjo>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Stk4<tm1.1Rjo> Stk3<tm1.1Rjo>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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