This Df(17)4Yey/+ mutant strain contains one copy of mouse Chromosome 17 with the targeted sequence deleted between, and including, the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene and ribosomal RNA processing 1 homolog B (S. cerevisiae) (Rrp1b) gene. Heterozygous mice are viable and fertile. This deleted region on the mouse chromosome is one of two regions orthologous to a deleted copy of human Chromosome 21 (Hsa21) which has been implicated in developmental cognitive disabilities like Down Syndrome (DS) and Monosomy 21. Dp(17)4Yey/+ lacks 19 genes on Chromosome 17 which share conserved synteny with the distal part of human 21q22.3, and is orthologous to genes on Hsa21. These mice exhibit Monosomy 21 related phenotypic defects including impaired contextual conditioning behavior, and abnormal spatial learning. These mice may be useful for understanding developmental and cognitive disabilities associated with Monosomy 21.

Development

Chromosome-engineering cassettes were inserted into mouse Chromosome 17 of 129S7/SvEvBrd-Hprt1^b-m2-derived AB2.2 embryonic stem (ES) cells, bracketing a span of approximately 1.1 Mb between, and including, the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene and ribosomal RNA processing 1 homolog B (S. cerevisiae) (Rrp1b) gene. The cassette placed at the proximal locus was targeted upstream of Abcg1 and contained an agouti transgene, a 3' portion of an HPRT minigene, a loxP site and puromycin resistance gene. The cassette placed at the distal locus was targeted downstream of the Rrp1b gene and contained a neomycin resistance gene, a loxP site, a 5' portion of an HPRT minigene, and a tyrosinase minigene. Double-targeted ES cells were transiently transfected with the cre recombinase expression vector pOG231. After subsequent selection of recombinants by using hypoxanthine aminopterin thymidine (HAT) media, correctly targeted ES cells, containing one copy of mouse Chromosome 17 with the targeted sequence deleted (Del), were injected into C57BL/6J-Tyr^c-Brd blastocysts. The resulting chimeric males were mated to C57BL/6J females and were subsequently backcrossed to 129/SvEv mice for at least 7 generations to produce a colony of Df(17)4Yey/+ mice. Upon arrival at The Jackson Laboratory these mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least one generation.

Control Suggestions

Wild-type from the colony

Approximate Controls

002448 129S1/SvImJ

Additional Information

Considerations for Choosing Controls

Selected References

Yu T; Clapcote SJ; Li Z; Liu C; Pao A; Bechard AR; Carattini-Rivera S; Matsui S; Roder JC; Baldini A; Mobley WC; Bradley A; Yu YE. 2010. Deficiencies in the region syntenic to human 21q22.3 cause cognitive deficits in mice. Mamm Genome 21(5-6):258-67. PubMed: 20512340 MGI: J:161398

View All References

Genetics

Del(17Abcg1-Rrp1b)5Yey

Allele Symbol: Del(17Abcg1-Rrp1b)5Yey

Allele Name	deletion, Chr 17, Y Eugene Yu 5
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Del(17)1Yey; Del(17Abcg1-Rrp1b)1Yey; Del4Yey; Df(17)1Yey; Df(Abcg1-Rrp1b)1Yey
Gene Symbol and Name	Del(17Abcg1-Rrp1b)5Yey, deletion, Chr 17, Y Eugene Yu 5
Gene Synonym(s)	Del(17Abcg1-Rrp1b)1Yey; Del(17Abcg1-Rrp1b)1Yey; deletion, Chr 17, Y Eugene Yu 1
Strain of Origin	129S7/SvEvBrd-Hprt
Chromosome	17
Molecular Note	Cre mediated chromosomal rearrangement between loxP sites in regions proximal to Abcg1 and distal to Rrp1b resulted in the deletion of the genes, including Abcg1 and Rrp1b, in this approximately 1.1 Mb region that is syntenic with the proximal part of human 21q22.3.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Ataxia (Movement) Defects
- Behavioral and Learning Defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Del(17Abcg1-Rrp1b)5Yey/+
129S.129S7-Del(17Abcg1-Rrp1b)5Yey

behavior/neurological phenotype

abnormal spatial learning
- after training, mice take longer to find the hidden platform in a Morris water maze test than wild-type mice

behavior/neurological phenotype
- mice exhibit normal cued conditioning and pain sensitivity

impaired contextual conditioning behavior
- mice exhibit reduced freezing in a 24-h and 72-h context test compared with wild-type mice

impaired swimming
- mice swim slower than wild-type mice

References

Yu T; Clapcote SJ; Li Z; Liu C; Pao A; Bechard AR; Carattini-Rivera S; Matsui S; Roder JC; Baldini A; Mobley WC; Bradley A; Yu YE. 2010. Deficiencies in the region syntenic to human 21q22.3 cause cognitive deficits in mice. Mamm Genome 21(5-6):258-67. PubMed: 20512340 MGI: J:161398

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Del(17Abcg1-Rrp1b)5Yey

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Strain(s) not available to companies or for-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

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