These mice possess loxP sites flanking exons 2 and 4 of the high mobility group nucleosomal binding domain 5 (Hmgn5) gene and may have applications in studies related to cancer progression.
Michael Bustin, NIH
These mice possess loxP sites on either side of exons 2 through 4 of the targeted gene, which code for the nucleosome binding domain. Female mice that are homozygous for this allele and male mice that are hemizygous for this X linked allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissue(s).
A FRT site flanked targeting vector containing a NEO selection cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 4, and another loxP site was inserted upstream of exon 2 of the targeted gene on the X chromosome. This construct was electroporated into (C57BL/6J x 129S4/SvJae)F1 derived v6.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on a C57BL/6J genetic background) expressing Flp recombinase. Mice that retained the floxed exons 2-4 were then bred to C57BL/6J mice for ten generations (and Flp transgene was removed) prior to sending males to The Jackson Laboratory Repository (see SNP results below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. This revealed 7 of 32 markers , one on chromosome 11 and two each on chromosomes 8, 13, and 15, that were not fixed for C57BL/6 allele-type (e.g.: segregating for 129S4 allele-type markers). These data suggest the mice sent to The Jackson Laboratory Repository were not sufficiently backcrossed onto the C57BL/6 genetic background to be considered congenic.
|Allele Name||targeted mutation 1.1, Michael Bustin|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Hmgn5, high-mobility group nucleosome binding domain 5|
|Strain of Origin||(C57BL/6J x 129S4/SvJae)F1|
|Molecular Note||A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette and a loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette and left exons 2 through 4 floxed.|
When maintaining a live colony, these mice can be bred as female homozygotes and male hemizygotes. The gene is X-linked.
When using the STOCK Hmgn5tm1.1Mbus/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #017298 in your Materials and Methods section.