Retinal vascular development and periendothelial cell recruitment appear normal in mice expressing the VEGF164 (Vegfatm3.1) isoform in contrast to impaired development in the VEGF120 and VEGF188 isoforms . This mutant mouse strain may be useful in studies of retinal vascular development.
Patricia D'Amore, Schepens Eye Research Institute
Heterozygote: Normal. Heterozygote phenotype is similar to the wild-type phenotype.
Homozygote: Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Retinal vascular development and periendothelial cell recruitment appear normal in mice expressing the VEGF164 isoform in contrast to impaired development in the VEGF120 and VEGF188 isoforms . This mutant mouse strain may be useful in studies of retinal vascular development.
A targeting vector was designed to insert a loxP-flanked neomycin cassette into the 3' UTR of a cDNA containing fused exons 4, 5, 7 and 8. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts The resulting chimeric animals were bred to mice of unknown background expressing a Cre recombinase driven by a Pgk promoter to remove the neo cassette leaving a loxP site downstream of exon 8. The VEGF 164 isoform lacks exon 6 and has intermediate binding properties. Offspring were crossed to C57BL/6 at least five times and then maintained by sibling mating. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 3.1, Peter Carmeliet|
|Gene Symbol and Name||Vegfa, vascular endothelial growth factor A|
|Promoter||Vegfa, vascular endothelial growth factor A, mouse, laboratory|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The floxed neo cassette was excised from Vegfatm3Pec by the in vivo expression of cre recombinase, leaving the isoform specific cDNA replacement intact. The resultant allele lacks exon 6 as well as splice sites and thereby produces a single isoform with intermediate solubility and receptor binding properties. The exclusive presence of this isoform was verified by quatitative RT-PCR.|
|Mutations Made By|| |
Dr. Peter Carmeliet, University of Leuven
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6.Cg-Vegfatm3.1Pec/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34405 in your Materials and Methods section.