These floxed mutant mice possess loxP sites flanking exon 1 of the Txnip gene. This strain may be useful for generating conditional mutations used in studies of metabolism, angiogenesis, and cancer.
Richard T Lee, Harvard Medical School
Exon 1 of this Txnip (thioredoxin interacting protein) targeted mutation strain is flanked by loxP sites. When bred to mice expressing cre recombinase under the control of a promoter of interest, selective excision of the floxed exon can be achieved, knocking out expression in a tissue/cell-specific manner. Homozygous floxed mice are viable and fertile.
For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in heart tissue (see Stock No. 005657), this mutant mouse strain may be useful in studies of myocardial energy metabolism.
A targeting construct incorporating a loxP-exon1-FRT-PGKneobpA-FRT-loxP sequence was introduced to J1 129S4/SvJae-derived embryonic stem (ES) cells. Resultant mice were crossed with 129Sv background animals expressing Gt(ROSA)26Sortm1(FLP1)Dym to excise the FRT-flanked neomycin cassette, leaving exon 1 flanked by loxP sites. This strain was maintained on a mixed 129 and C57BL/6 genetic background by the donating laboratory, and crossed with a cardiac-specific C57BL/6-129 Mer-Cre-Mer strain. This non-germline Cre has been bred away from the line maintained at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Richard T Lee|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Txnip, thioredoxin interacting protein|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A loxP site was inserted 168 bp upstream of the first ATG codon in exon 1 and an frt flanked neo cassette and second loxP were inserted downstream of exon 1 via homologous recombination. The neo cassette was removed via in vivo flp-mediated recombination.|
|Mutations Made By|| |
Richard Lee, Harvard Medical School