These LoxP membralin mice possess loxP sites on either side of exons 2 through 4 of the ORF61 (open reading frame 61) gene and and have applications in studies related to spinal muscular atrophy and motor neuron diseases.
Dongxian Zhang, Sanford-Burnham Med. Res. Inst.
These mice possess loxP sites on either side of exons 2 through 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissues.
A loxP/FRT site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 4. This construct was electroporated into into iTL1 BA1 (C57BL/6 x 129/SvEv) hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then crossed to FLP recombinase expressing mice on the C57BL/6 background to remove the PGK-Neo cassette. Heterozygotes were intercrossed to generate homozygotes.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that this strain was on a mixed genetic background.
|Allele Name||targeted mutation 1.1, inGenious Targeting Laboratory|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Tmem259, transmembrane protein 259|
|Strain of Origin||(C57BL/6NTac x 129S6/SvEvTac)F1|
|Molecular Note||A loxP/FRT site flanked targeting vector containing PGK-Neo selection cassette was utilized in the construction of this allele. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette.|
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129-Tmem259tm1.1Itl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016574 in your Materials and Methods section.