JAX Mouse Strain Datasheet - 016116

STOCK Wap^tm2(rtTA)Kuw/J

Stock No:: 016116

Targeted Mutation

Cryo Recovery

Overview

These mice contain a reverse tetracycline-controlled transactivator (rtTA) protein in the 5' untranslated region of the whey acidic protein gene. Theses Wap-rtTA mice are useful in regulating the expression of proteins (including oncoproteins) in mammary epithelium.

Donating Investigator

Dr. Kay-Uwe Wagner, University of Nebraska Medical Center

Genetic overview

Genetic Background	Generation

Wap^tm2(rtTA)Kuw

Allele Type	Gene Symbol	Gene Name
Targeted (Transactivator)	Wap	whey acidic protein

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Research Applications

Cell Biology Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

These mice contain a reverse tetracycline-controlled transactivator (rtTA) protein in the 5' untranslated region of the whey acidic protein (Wap) gene. Homozygous mice are viable, fertile, and normal in size. WAP, expressed late during pregnancy and during lactation in the mammary gland, is the principal whey protein found in rodent milk. When mated to a mutant strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TetO), the Wap-rtTA allele mediates expression of TetO-responder genes in the developing mammary gland upon administration of doxycycline (dox). Since expression of the endogenous WAP locus is elevated during pregnancy and lactation, the expression of TetO responder genes is particularly high during these stages of mammary gland development. The Wap-rtTA mice are useful to express exogenous proteins including teto-regulated oncogenes in the mammary epithelium.

Development

A targeting vector was designed to place a reverse tetracycline-controlled transactivator (rtTA) protein, followed by a loxP-flanked neomycin resistance (neo) cassette, in the 5' untranslated region of the whey acidic protein (Wap) gene. The construct was electroporated into 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL6NCrl blastocysts and the resulting chimeric males were bred to C57BL6NCrl females. Offspring were then crossed to STOCK-Tg(MMTV-Cre)1Mam/J transgenic mice (Stock No. 003551) to remove the neo cassette. The resulting double positive females were then bred to wildtype males to remove the Cre transgene. These mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.

Expression Data

Expressed Gene	rtTA, reverse tetracycline-controlled transactivator, E. coli
Site of Expression

Control Suggestions

Approximate Controls

005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Creamer BA; Triplett AA; Wagner KU. 2009. Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging. Genesis 47(4):234-45. PubMed: 19208431 MGI: J:148684

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Genetics

Wap^tm2(rtTA)Kuw

Allele Symbol: Wap^tm2(rtTA)Kuw

Allele Name	targeted mutation 2, Kay Uwe Wagner
Allele Type	Targeted (Transactivator)
Allele Synonym(s)
Gene Symbol and Name	Wap, whey acidic protein
Gene Synonym(s)
Expressed Gene	rtTA, reverse tetracycline-controlled transactivator, E. coli
Strain of Origin	129X1/SvJ
Chromosome	11
Molecular Note	The coding sequence for rtTA was inserted into the 5'-UTR along with a floxed neomycin selection cassette. The neo selection cassette was eliminated in founder line 7A3 by crossing properly targeted mice to Tg(MMTV-cre)1Mam transgenic animals. When mice were given doxycycline, transgene expression was restricted to mammary epithelial cells as determined by crosses with two different rtTA reporter strains.
Mutations Made By	Dr. Kay-Uwe Wagner, University of Nebraska Medical Center

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cell Biology Research
- Protein Processing
Research Tools
- Cancer Research
  - Tetop Tet System
- Genetics Research
  - Mutagenesis and Transgenesis: Tetop Tet System

References

Creamer BA; Triplett AA; Wagner KU. 2009. Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging. Genesis 47(4):234-45. PubMed: 19208431 MGI: J:148684

Additional - Wap^tm2(rtTA)Kuw related

Creamer BA; Sakamoto K; Schmidt JW; Triplett AA; Moriggl R; Wagner KU. 2010. Stat5 promotes survival of mammary epithelial cells through transcriptional activation of a distinct promoter in Akt1. Mol Cell Biol 30(12):2957-70. PubMed: 20385773 MGI: J:162571
Schmidt JW; Wehde BL; Sakamoto K; Triplett AA; Anderson SM; Tsichlis PN; Leone G; Wagner KU. 2014. Stat5 regulates the phosphatidylinositol 3-kinase/Akt1 pathway during mammary gland development and tumorigenesis. Mol Cell Biol 34(7):1363-77. PubMed: 24469394 MGI: J:213526

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Wap<tm2(rtTA)Kuw>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

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Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Waptm2(rtTA)Kuw

Allele Symbol: Waptm2(rtTA)Kuw

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

References

Additional - Waptm2(rtTA)Kuw related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Wap^tm2(rtTA)Kuw

Allele Symbol: Wap^tm2(rtTA)Kuw

Additional - Wap^tm2(rtTA)Kuw related